Bhisey R A, Ramchandani A G, D'Souza A V, Borges A M, Notani P N
Carcinogenesis Division, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai, India.
Int J Cancer. 1999 Nov 26;83(5):679-84. doi: 10.1002/(sici)1097-0215(19991126)83:5<679::aid-ijc19>3.0.co;2-e.
Carcinogenicity of pan masala, a dry powdered chewing mixture of areca nut, catechu, lime, spices and flavoring agents was evaluated by means of the long-term animal bio-assay 6- to 7-week old male and female S/RVCri mice were divided randomly into intermediate and lifetime exposure groups and fed normal diet without pan masala-(zero dose) or diet containing 2.5% and 5% pan masala. Animals in the intermediate-exposure group (n = 10/gender/dose group) were killed after 6, 12 or 18 months of treatment, while those in the lifetime-exposure group (n = 54/gender/dose group) were killed when moribund or at the termination of the experiment at 24 months. Several tissues were processed for histopathological examination. The body weight and survival rate of mice fed pan masala were lower than that of the controls. Histopathological observations of tissues from control animals did not reveal any neoplastic alterations. However, lifetime feeding of pan masala induced adenoma of the liver, stomach, prostate and sebaceous glands, also forestomach papilloma, liver hamartoma, hepatoma and hemangioma, carcinoma of the forestomach, adenocarcinoma of the lung and liver, and testicular lymphoma. Neoplastic lesions appeared mainly in the liver (n = 13), stomach (n = 3) and lung (n = 8). Lung adenocarcinoma, the most frequent malignant tumor type, was observed in 2/120 mice in the intermediate-exposure group and in 8/216 animals in the lifetime-exposure group. Statistical analysis of tumor-induction data revealed a significant dose-related increase in lung adenocarcinomas but not in liver and stomach neoplasms indicating that lung is the major target tissue for the carcinogenic action of pan masala.
通过长期动物生物测定评估了一种干粉状咀嚼混合物——帕安玛萨拉（pan masala）的致癌性，该混合物由槟榔、儿茶、石灰、香料和调味剂组成。将6至7周龄的雄性和雌性S/RVCri小鼠随机分为中期暴露组和终身暴露组，分别喂食不含帕安玛萨拉的正常饮食（零剂量）或含有2.5%和5%帕安玛萨拉的饮食。中期暴露组（每组性别各10只/剂量组）在治疗6、12或18个月后处死，而终身暴露组（每组性别各54只/剂量组）在濒死时或在24个月实验结束时处死。对多个组织进行了组织病理学检查。喂食帕安玛萨拉的小鼠体重和存活率低于对照组。对照动物组织的组织病理学观察未发现任何肿瘤性改变。然而，终身喂食帕安玛萨拉会诱发肝脏、胃、前列腺和皮脂腺腺瘤，还有前胃乳头状瘤、肝脏错构瘤、肝癌和血管瘤，前胃癌、肺和肝癌腺癌以及睾丸淋巴瘤。肿瘤性病变主要出现在肝脏（13例）、胃（3例）和肺（8例）。肺腺癌是最常见的恶性肿瘤类型，在中期暴露组的120只小鼠中有2只出现，在终身暴露组的216只动物中有8只出现。肿瘤诱导数据的统计分析显示，肺腺癌有显著的剂量相关增加，但肝脏和胃部肿瘤没有，这表明肺是帕安玛萨拉致癌作用的主要靶组织。
