使用分枝杆菌生长指示管（MGIT）和罗-琴培养基检测临床标本中的分枝杆菌。

Detection of mycobacteria in clinical specimen using the mycobacteria growth indicator tube (MGIT) and the Lowenstein Jensen medium.

作者信息

Levidiotou S, Papamichael D, Gessouli E, Golegou S, Anagnostou S, Galanakis E, Papadopoulou C, Antoniadis G

机构信息

Department of Microbiology, Medical School, University of Ioannina, Greece.

出版信息

Microbiol Res. 1999 Sep;154(2):151-5. doi: 10.1016/S0944-5013(99)80008-1.

DOI:10.1016/S0944-5013(99)80008-1

PMID:10522382

Abstract

The recovery rates of mycobacteria strains isolated from 1200 clinical specimens using the mycobacteria growth indicator tube (MGIT) system and the conventional Lowenstein Jensen medium (LJ) were assessed. Of the 87 mycobacterial isolates recovered, 54 belonged to the M. tuberculosis complex (MTB) and 33 to the non-tuberculosis complex (NTM). MGIT recovered 78 (89.65%) mycobacteria isolates (51 MTB (94.44%) and 27 NTM (81.81%) and LJ recovered 70 (80.46%) mycobacteria isolates (49 MTB (90.74%) and 21 NTM (63.63%). Sixty one (70.1%) of the total mycobacteria isolates were recovered with both systems (46 (85.2%) MTB and 15 (45.5%) NTM). No significant difference was found between MGIT and LJ (p > 0.05) in both MTB and NTM recoveries. The average detection time for MTB was significantly shorter with MGIT than with LJ, in both the smear-positive specimens (8 vs 30 days: p < 0.0001) and smear-negative specimens (15 vs 30 days: p < 0.001). The average detection time of NTM was also shorter for MGIT (15 vs 30 days: p < 0.0001). However, the contamination rate was higher in MGIT (8.5%) than in LJ (3%). The results suggest that the use of MGIT contributes to a more rapid and effective diagnosis of mycobacterial infections particularly when combined with the classical LJ.

摘要

评估了使用分枝杆菌生长指示管（MGIT）系统和传统的罗-琴培养基（LJ）从1200份临床标本中分离出的分枝杆菌菌株的回收率。在回收的87株分枝杆菌分离株中，54株属于结核分枝杆菌复合群（MTB），33株属于非结核分枝杆菌复合群（NTM）。MGIT回收了78株（89.65%）分枝杆菌分离株（51株MTB（94.44%）和27株NTM（81.81%）），LJ回收了70株（80.46%）分枝杆菌分离株（49株MTB（90.74%）和21株NTM（63.63%））。两种系统共回收了61株（70.1%）分枝杆菌分离株（46株（85.2%）MTB和15株（45.5%）NTM）。在MTB和NTM的回收率方面，MGIT和LJ之间未发现显著差异（p>0.05）。在涂片阳性标本（8天对30天：p<0.0001）和涂片阴性标本（15天对30天：p<0.001）中，MGIT检测MTB的平均时间均显著短于LJ。MGIT检测NTM的平均时间也较短（15天对30天：p<0.0001）。然而，MGIT的污染率（8.5%）高于LJ（3%）。结果表明，使用MGIT有助于更快速、有效地诊断分枝杆菌感染，特别是与经典的LJ联合使用时。