Lorenz S A, Moy M A, Dolan A R, Wood T D
Department of Chemistry, Natural Sciences Complex, State University of New York at Buffalo, Buffalo, NY 14260-3000, USA.
Rapid Commun Mass Spectrom. 1999;13(21):2098-102. doi: 10.1002/(SICI)1097-0231(19991115)13:21<2098::AID-RCM759>3.0.CO;2-O.
Fourier transform mass spectrometry (FTMS), long-known for its capabilities in structural characterization of molecules, is an emerging tool in quantification, and quantification methods using external and internal standards with electrospray ionization (ESI) FTMS have recently been demonstrated. Here, commercial ESI-FTMS is used to quantify the opioid pentapeptide methionine enkephalin using an internal standard. Linear working curves over three orders of magnitude are obtained using the internal standard, an improvement of one order of magnitude over the previous external standard ESI-FTMS quantification method for enkephalins. Low coefficients of variation (generally <6%) are observed, and inter-day and intra-day assays are compared and found to possess similar linearity and precision. The high mass accuracy advantage of FTMS can be exploited to give molecular specificity. Efforts to improve mass accuracy using internal mass calibration generally provide mass accuracies within 2.5 ppm.
傅里叶变换质谱（FTMS）长期以来以其在分子结构表征方面的能力而闻名，如今它正成为一种新兴的定量工具，并且最近已经展示了使用外部和内部标准以及电喷雾电离（ESI）FTMS的定量方法。在此，使用商用ESI-FTMS并借助内标对阿片类五肽甲硫氨酸脑啡肽进行定量。使用内标获得了跨越三个数量级的线性工作曲线，相较于之前用于脑啡肽的外标ESI-FTMS定量方法，在数量级上有一个提升。观察到变异系数较低（通常<6%），并且对日间和日内测定进行了比较，发现它们具有相似的线性和精密度。FTMS的高质量精度优势可用于实现分子特异性。使用内部质量校准来提高质量精度的努力通常能使质量精度达到2.5 ppm以内。
