二氧化碳气腹对大鼠实体结肠癌生长的影响。

The effect of CO2 pneumoperitoneum on the growth of a solid colon carcinoma in rats.

作者信息

Pauwels M, Lauwers P, Hendriks J, Hubens A, Eyskens E, Hubens G

机构信息

Laboratory of Experimental Surgery, University of Antwerp, Wilrijkstraat 10, 2650 Edegem, Antwerp, Belgium.

出版信息

Surg Endosc. 1999 Oct;13(10):998-1000. doi: 10.1007/s004649901155.

DOI:10.1007/s004649901155

PMID:10526035

Abstract

BACKGROUND

In order to investigate the effect of carbon dioxide (CO(2)) pneumoperitoneum on solid colon carcinomas, we used a colon anastomosis tumor model in 30 male syngeneic WAG rats, which were divided, at random into three groups.

METHODS

In all rats, 10(6) CC531 S colon carcinoma cells were injected as an enema into the colon. Subsequently, a transection and a reanastomosis of the colon descendens was performed via laparotomy. After 2 weeks, group 1 (n = 10) was anesthetized as an anesthesia control group. Group 2 (n = 10) had a laparotomy that was closed after 20 min. In group 3 (n = 10), a CO(2) pneumoperitoneum of </=6 mmHg was created and maintained during 20 min. After 2 weeks, all rats were killed, and total tumor weight and volume was measured.

RESULTS

At necroscopy tumor growth was found in 16 rats (group 1: six; group 2: five; group 3: five). No difference in tumor growth (weight or volume) was found between the three groups.

CONCLUSION

In our solid colon carcinoma model, CO(2) pneumoperitoneum did not enhance tumor growth.

摘要

背景

为了研究二氧化碳（CO₂）气腹对实体结肠癌的影响，我们在30只雄性同基因WAG大鼠中使用结肠吻合肿瘤模型，将其随机分为三组。

方法

对所有大鼠，经灌肠向结肠内注入10⁶个CC531 S结肠癌细胞。随后，通过剖腹术对降结肠进行横断和再吻合。2周后，第1组（n = 10）作为麻醉对照组进行麻醉。第2组（n = 10）进行剖腹术，20分钟后关闭腹腔。第3组（n = 10）建立并维持≤6 mmHg的CO₂气腹20分钟。2周后，处死所有大鼠，测量肿瘤总重量和体积。