Chiu L C, Wan J M
Department of Zoology, The University of Hong Kong, People's Republic of China.
Cancer Lett. 1999 Oct 18;145(1-2):17-27. doi: 10.1016/s0304-3835(99)00224-4.
Dietary polyunsaturated fatty acids (PUFAs) have been reported as a potential group of natural products which modulate tumor cell growth. In present study, eicosapentaenoic acid (EPA) was found to inhibit proliferation of human leukemic HL-60 and K-562 cells in vitro. EPA arrested cell cycle progression at G0/G1 phase, and induced necrosis in both HL-60 and K-562 cells. However, EPA induced apoptosis only in HL-60 but not K-562 cells. Also, bcl-2 protein expression was downregulated in much greater extent than that of bax showing that depression of bcl-2 might be an important step during the EPA-induced apoptosis in HL-60 cells.
据报道，膳食多不饱和脂肪酸（PUFAs）是一类具有调节肿瘤细胞生长潜力的天然产物。在本研究中，发现二十碳五烯酸（EPA）在体外可抑制人白血病HL-60和K-562细胞的增殖。EPA使细胞周期进程停滞在G0/G1期，并诱导HL-60和K-562细胞发生坏死。然而，EPA仅诱导HL-60细胞凋亡，而不诱导K-562细胞凋亡。此外，bcl-2蛋白表达下调的程度远大于bax，这表明bcl-2的抑制可能是EPA诱导HL-60细胞凋亡过程中的重要一步。
