Department of Radiotherapy, University of Rostock, Rostock, Germany.
Strahlenther Onkol. 2011 Feb;187(2):127-34. doi: 10.1007/s00066-010-2166-6. Epub 2011 Jan 18.
The aim of this study was to evaluate whether the omega-3 polyunsaturated fatty acid cis-5,8,11,14,17-eicosapentanoic acid (EPA) can enhance the radiosensitivity of different human tumor cell lines.
Colon adenocarcinoma cells HT-29, and two glioblastoma multiforme tumor cells T98G and U251 were cultured under standard conditions. Cell growth was observed during administration with different concentrations of EPA, using it as the free fatty acid dissolved in ethanol or bound to bovine serum albumin. To investigate the influence of EPA (free and bound) on radiosensitivity, tumor cells were pretreated 30 minutes or 24 hours prior to irradiation with the fatty acid. Cell survival was measured by colony-forming assays.
When combined with irradiation, incubation with EPA was found to result in enhanced radiosensitivity with substantial variation: while there was strong radiosensitization for HT-29 and U251 cells, almost no effect for T98G cells was observed. A marked radiosensitization was clearly dependent on the treatment schedule.
The observations suggest that EPA is not only a nutritional adjuvant but also may be a potential candidate to enhance the efficacy of irradiation on human cancer cells.
本研究旨在评估ω-3 多不饱和脂肪酸顺式-5,8,11,14,17-二十碳五烯酸（EPA）是否能增强不同人类肿瘤细胞系的放射敏感性。
结肠腺癌细胞 HT-29 以及两种多形性胶质母细胞瘤细胞 T98G 和 U251 在标准条件下培养。用不同浓度的 EPA 作为溶于乙醇的游离脂肪酸或与牛血清白蛋白结合的形式进行处理，观察细胞生长情况。为了研究 EPA（游离和结合）对放射敏感性的影响，在用脂肪酸预处理 30 分钟或 24 小时后进行照射。通过集落形成实验测量细胞存活率。
与照射联合使用时，发现 EPA 的孵育导致放射敏感性增强，差异很大：HT-29 和 U251 细胞表现出强烈的放射增敏作用，而 T98G 细胞几乎没有作用。明显的放射增敏作用显然依赖于治疗方案。
这些观察结果表明，EPA 不仅是一种营养佐剂，而且可能是增强人类癌细胞放射治疗效果的潜在候选药物。
