Boeker M, Pabst R, Rothkötter H J
Center of Anatomy, Medical School of Hannover, Germany.
Immunobiology. 1999 Sep;201(1):74-87. doi: 10.1016/S0171-2985(99)80048-5.
Research on the pig's immune system is not only of general biological interest; the pig is also becoming more important as a large animal model in human biomedical research, e.g. as a donor for xeno-transplantation. With the increasing panel of monoclonal antibodies against porcine lymphocyte markers it is possible to gain more insight into the distribution and phenotype of lymphocyte subpopulations in the pig. In this study we investigated B cells (surface IgG: sIgG, sIgM and sIgA) and T cells (CD2, CD4, CD8, 8/1, MAC320) in the peripheral blood (pBL), thymus, spleen, tonsil, mesenteric and inguinal lymph nodes (mLN, iLN), jejunal and ileal Peyer's patches (jejPP, ilPP) in Göttingen minipigs. A flow cytometric technique was employed which enabled three color indirect immunofluorescence. B cell stained for surface IgG and surface IgA were found only in small percentages. Surface IgM positive cells were distributed at higher rates, with up to 24.9% in the iLN. Up to 64.2% of CD4+ and up to 73.1% of CD8+ cells were observed in the thymus. Most of the CD4+ cells were CD4/CD8 double positive cells. These cells were mostly triple positive in combination with CD2. A larger fraction of CD2- were CD8- which are taken to be NK cells. MAC320, a marker for a subtype of gamma/delta T cells, was predominantly found on cells in the pBL. The standardized flow cytometric technique produced comparable data on the distribution of major lymphocyte subpopulations in the blood and different lymphoid organs of the pig. The results provide a basis for future studies using the pig as animal model.
对猪免疫系统的研究不仅具有一般生物学意义；猪作为人类生物医学研究中的大型动物模型也变得越来越重要，例如作为异种移植的供体。随着针对猪淋巴细胞标志物的单克隆抗体种类不断增加，有可能更深入地了解猪淋巴细胞亚群的分布和表型。在本研究中，我们调查了哥廷根小型猪外周血（pBL）、胸腺、脾脏、扁桃体、肠系膜和腹股沟淋巴结（mLN、iLN）、空肠和回肠派伊尔结（jejPP、ilPP）中的B细胞（表面IgG：sIgG、sIgM和sIgA）和T细胞（CD2、CD4、CD8、8/1、MAC320）。采用了一种流式细胞术技术，该技术能够进行三色间接免疫荧光检测。仅发现小百分比的B细胞表面IgG和表面IgA呈阳性染色。表面IgM阳性细胞的分布率较高，在iLN中高达24.9%。在胸腺中观察到高达64.2%的CD4+细胞和高达73.1%的CD8+细胞。大多数CD4+细胞是CD4/CD8双阳性细胞。这些细胞大多与CD2结合呈三阳性。较大比例的CD2阴性细胞是CD8阴性细胞，被认为是自然杀伤细胞。MAC320是γ/δT细胞亚型的标志物，主要在pBL中的细胞上发现。标准化的流式细胞术技术产生了关于猪血液和不同淋巴器官中主要淋巴细胞亚群分布的可比数据。这些结果为未来将猪用作动物模型的研究提供了基础。
