Caro M R, Gallego M C, Buendía A J, Navarro E, Navarro J A
Departamento de Patologia Animal (Microbiologia e Immunologia), Facultad de Veterinaria, Universidad de Murcia, Spain.
Res Vet Sci. 1998 Sep-Oct;65(2):145-8. doi: 10.1016/s0034-5288(98)90166-7.
Monoclonal antibodies (mAbs) against bovine leukocyte antigens specific for T cells (CD2, CD4, CD8 and gammadelta receptor) and B cells (surface IgM) were used in samples from one week and one-, three- and seven-month-old goats to study the evolution of lymphocyte subsets by flow cytometry in peripheral blood and the lymphoid organs: thymus, jejunal (JPP) and ileal (IPP) Peyer's patches, spleen and mesenteric lymph nodes. An increase in the values of alpha/beta receptor T cells with age was recorded whereas the gammadelta receptor T cells fell in number. In peripheral blood and in all tissues, except IPP the values for B cells (sIgM+) were low. The CD4+ and CD8+ cells predominated in JPP while B cells were the most important subpopulation in IPP. In the spleen, as in JPP, the CD4/CD8 ratio was less than one and the gammadelta T cells values were high. In mesenteric lymph nodes, CD8+ and B(sIgM) cells predominated in the youngest animals.
针对T细胞（CD2、CD4、CD8和γδ受体）和B细胞（表面IgM）特异性的牛白细胞抗原的单克隆抗体（mAbs）被用于来自1周龄以及1个月、3个月和7月龄山羊的样本，以通过流式细胞术研究外周血和淋巴器官（胸腺、空肠派伊尔结（JPP）、回肠派伊尔结（IPP）、脾脏和肠系膜淋巴结）中淋巴细胞亚群的演变。记录到α/β受体T细胞的值随年龄增加，而γδ受体T细胞数量减少。在外周血和除IPP外的所有组织中，B细胞（sIgM+）的值较低。CD4+和CD8+细胞在JPP中占主导，而B细胞是IPP中最重要的亚群。在脾脏中，与JPP一样，CD4/CD8比率小于1，γδ T细胞的值较高。在肠系膜淋巴结中，CD8+和B（sIgM）细胞在最年幼的动物中占主导。
