Jarrold B B, Bacon W L, Velleman S G
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA.
Atherosclerosis. 1999 Oct;146(2):299-308. doi: 10.1016/s0021-9150(99)00154-9.
The temporal and spatial distribution, and relative levels of the proteoglycan decorin and collagen type I were examined during the progression of atherosclerosis in the dorsal aortas of Japanese quail selected for cholesterol induced atherosclerosis. The quail were placed on either a control or 0.5% added cholesterol diet at approximately 16 weeks of age. Dorsal aortas were collected at 1- or 2-week intervals over a 15-week period after initiating cholesterol feeding. Biochemical analysis for decorin and collagen type I showed that both increased in the cholesterol-fed birds compared to control-fed birds beginning at 9 weeks and continued through the duration of the study. Through immunohistochemical staining for decorin and collagen type I, the spatial localization of decorin and collagen type I in control and less severe plaques in cholesterol-fed birds was most prominent in the arterial adventitia. However, in severe atherosclerotic plaques, decorin was localized in foam cell regions and collagen type I was found surrounding the foam cell regions where decorin accumulated. These results demonstrated a localization of decorin in the core of the atherosclerotic plaque foam cell region with collagen type I being located on the plaque surface.
在因胆固醇诱导动脉粥样硬化而被选择的日本鹌鹑的胸主动脉中，研究了蛋白聚糖核心蛋白聚糖和I型胶原蛋白在动脉粥样硬化进展过程中的时空分布及相对水平。鹌鹑在大约16周龄时被置于对照饮食或添加0.5%胆固醇的饮食中。在开始喂食胆固醇后的15周内，每隔1周或2周收集胸主动脉。对核心蛋白聚糖和I型胶原蛋白的生化分析表明，与对照喂养的鹌鹑相比，喂食胆固醇的鹌鹑从第9周开始两者均增加，并持续到研究结束。通过对核心蛋白聚糖和I型胶原蛋白进行免疫组织化学染色，在对照和胆固醇喂养鹌鹑中较轻的斑块中，核心蛋白聚糖和I型胶原蛋白在动脉外膜中的空间定位最为明显。然而，在严重的动脉粥样硬化斑块中，核心蛋白聚糖定位于泡沫细胞区域，而I型胶原蛋白则在核心蛋白聚糖积聚的泡沫细胞区域周围被发现。这些结果表明，核心蛋白聚糖定位于动脉粥样硬化斑块泡沫细胞区域的核心，而I型胶原蛋白位于斑块表面。
