Küçüktülü U, Alhan E, Erçin C, Cinel A, Calik A
Department of General Surgery, Karadeniz Technical University School of Medicine, Trabzon, Turkey.
Eur J Surg. 1999 Sep;165(9):891-6. doi: 10.1080/11024159950189410.
To find out the effects of the octreotide on the course of acute pancreatitis in rats.
Prospective laboratory study.
Medical school, Turkey
184 Sprague-Dawley rats, 120 of which were randomly allocated into 8 groups of 15 each for the survival study, and the remainder of which were randomly allocated into 8 groups of 8 rats each for assessment of biochemical variables and histological score.
The same 8 groups were used for the two parts of the study: saline alone (control), octreotide alone (control), oedematous pancreatitis induced by cerulein with and without octreotide, moderate pancreatitis induced by low-dose glycodeoxycholic acid and cerulein with and without octreotide, and severe pancreatitis induced by high-dose glycodeoxycholic acid and cerulein with and without octreotide.
Mortality, results of biochemical tests, and histological score.
No rats in the control groups died. Of those with oedematous pancreatitis 1 died that had not been given octreotide (7%) and 2 that had (13%). In the moderate pancreatitis groups 4 that had not been given octreotide died (27%) compared with one that had (7%). In the severe pancreatitis group 7 that had not had octreotide died (46%) compared with 6 that had (40%). Octreotide caused a reduction in serum amylase and lactate dehydrogenase activity in all groups, but reduced aspartate aminotransferase only in those rats with moderate pancreatitis. It prevented hypocalcaemia in rats with severe pancreatitis, but had no effect on serum electrolyte concentrations, alkaline phosphatase activity, or blood gas analyses. Rats with moderate pancreatitis that had been given octreotide had less tissue oedema, acinar necrosis, and inflammatory cell infiltration. In those with severe pancreatitis there was less tissue oedema but more acinar necrosis.
If octreotide is given early in the course of the disease it may result in improved outcome, but it seems to be ineffective in severe pancreatitis in which acinar necrosis is already established.
探讨奥曲肽对大鼠急性胰腺炎病程的影响。
前瞻性实验室研究。
土耳其医学院
184只Sprague-Dawley大鼠，其中120只随机分为8组，每组15只用于生存研究，其余随机分为8组，每组8只用于评估生化指标和组织学评分。
研究的两个部分使用相同的8组：单纯生理盐水（对照组）、单纯奥曲肽（对照组）、有或无奥曲肽的雨蛙肽诱导的水肿性胰腺炎、低剂量甘氨脱氧胆酸和雨蛙肽诱导的中度胰腺炎且有或无奥曲肽、高剂量甘氨脱氧胆酸和雨蛙肽诱导的重症胰腺炎且有或无奥曲肽。
死亡率、生化检测结果和组织学评分。
对照组无大鼠死亡。在水肿性胰腺炎组中，未给予奥曲肽的有1只死亡（7%），给予奥曲肽的有2只死亡（13%）。在中度胰腺炎组中，未给予奥曲肽的有4只死亡（27%），给予奥曲肽的有1只死亡（7%）。在重症胰腺炎组中，未给予奥曲肽的有7只死亡（46%），给予奥曲肽的有6只死亡（40%）。奥曲肽使所有组的血清淀粉酶和乳酸脱氢酶活性降低，但仅使中度胰腺炎大鼠的天冬氨酸转氨酶降低。它预防了重症胰腺炎大鼠的低钙血症，但对血清电解质浓度、碱性磷酸酶活性或血气分析无影响。给予奥曲肽的中度胰腺炎大鼠组织水肿、腺泡坏死和炎性细胞浸润较少。在重症胰腺炎大鼠中，组织水肿较少但腺泡坏死较多。
如果在疾病早期给予奥曲肽，可能会改善预后，但在腺泡坏死已经形成的重症胰腺炎中似乎无效。
