Ozer Cakir Ozlem, Esen Hasan, Toker Aysun, Ataseven Huseyin, Demir Ali, Polat Hakki
Department of Gastroenterology and Hepatology, Konya Education and Research Hospital Konya, Turkey.
Department of Pathology, Meram School of Medicine, Necmettin Erbakan University Konya, Turkey.
Int J Clin Exp Med. 2015 Oct 15;8(10):17551-64. eCollection 2015.
Research continues to develop novel therapeutic modalities that particularly focus on the pathogenesis of acute pancreatitis. This study aimed to assess the effects of diclofenac sodium and octreotide, alone or in combination, on pancreatic enzymes, pancreatic myeloperoxidase activity, histopathology and apoptosis of pancreas cells, using a model of experimentally induced acute pancreatitis.
We aimed to demonstrate effects of diclofenac sodium, octreotide and their combined use on pancreatic enzymes, activity of pancreatic myeloperoxidase (MPO) activity, histopathology and apoptosis of pancreas on treatment of caerulin-induced experimental acute pancreatitis.
Caerulin-induced acute pancreatitis model was created using a total of 58 male BALB-C mice of 25 gr in seven groups. Serum amylase, lipase levels and pancreatic myeloperoxidase activity were examined as well as apoptotic values in pancreatic acinar cells through TUNNEL method. Histopathology of pancreas was evaluated for presence of edema, hemorrhage, parenchymal necrosis, fat necrosis, leukocyte infiltration, and fibrosis.
In the diclofenac sodium group, apoptotic values in the pancreatic acinar cells were found to be statistically lower than in the acute pancreatitis group in terms of parenchymal necrosis and hemorrhage scores (P = 0.007, P = 0.002, and P = 0.052, respectively). No statistically significant differences were found in serum level of amylase, lipase, pancreatic myeloperoxidase activity and the other histopathological scores (P > 0.05).
Diclofenac sodium, a cost-effective agent with a favorable side-effect profile, may represent a novel therapeutic agent for the treatment of acute pancreatitis. Findings of this study suggest a better efficacy for diclofenac sodium monotherapy as compared to octreotide alone or octreotide/diclofenac combination.
研究不断开发新的治疗方法，尤其关注急性胰腺炎的发病机制。本研究旨在利用实验性诱导急性胰腺炎模型，评估双氯芬酸钠和奥曲肽单独或联合使用对胰腺酶、胰腺髓过氧化物酶活性、胰腺组织病理学及胰腺细胞凋亡的影响。
我们旨在证明双氯芬酸钠、奥曲肽及其联合使用对蛙皮素诱导的实验性急性胰腺炎治疗中胰腺酶、胰腺髓过氧化物酶（MPO）活性、胰腺组织病理学及胰腺细胞凋亡的影响。
使用总共58只25克重的雄性BALB - C小鼠，分为七组，建立蛙皮素诱导的急性胰腺炎模型。检测血清淀粉酶、脂肪酶水平和胰腺髓过氧化物酶活性，并通过TUNNEL法检测胰腺腺泡细胞的凋亡值。评估胰腺组织病理学，观察是否存在水肿、出血、实质坏死、脂肪坏死、白细胞浸润和纤维化。
在双氯芬酸钠组中，就实质坏死和出血评分而言，胰腺腺泡细胞的凋亡值在统计学上低于急性胰腺炎组（分别为P = 0.007、P = 0.002和P = 0.052）。血清淀粉酶水平、脂肪酶、胰腺髓过氧化物酶活性及其他组织病理学评分未见统计学显著差异（P > 0.05）。
双氯芬酸钠是一种具有良好副作用谱的经济有效的药物，可能代表一种治疗急性胰腺炎的新型治疗药物。本研究结果表明，与单独使用奥曲肽或奥曲肽/双氯芬酸联合使用相比，双氯芬酸钠单一疗法疗效更佳。
