Sánchez Manuel J, Landa García J I, Seco Gil J L, Velasco Oses A, Coma del Corral M J, Vaquero Puerta C, Santamaría García J L
Laboratorios de Cirugía Experimental, Hospital Gral. Yagüe, Burgos.
Rev Esp Enferm Dig. 1997 Feb;89(2):101-15.
The effect of octreotide in acute experimental pancreatitis was examined.
Acute pancreatitis was induced in 70 male Wistar rats by retrograde injection of 5% sodium taurocholate into the pancreatic duct. Octreotide (50 micrograms = 0.5 ml) or isotonic saline was administered subcutaneously every six hours for 24 hours, beginning at the time of induction of pancreatitis (groups 1 and 2) or 12 hours later (groups 3 and 4). Six hours after the last dose of octreotide or saline, the rats were sacrificed. Ten additional rats were used for basal biochemical determination. For the analysis of survival, another 10 rats were included. The survival rate, serum concentrations of amylase, lipase and lactate dehydrogenase (LDH) and morphometric studies (total area of the pancreas and percentage necrosis) were examined. Statistical analysis involved Student's t test, ANOVA and the Mantel-Haenszel test.
No significant differences existed among the groups with respect to survival, morphometric analysis or biochemical determinations, except for a minor increase in LDH levels in the group treated with octreotide at the time of induction.
Octreotide administration has almost no impact on mortality in experimental severe acute pancreatitis. Biochemical and morphometric changes are minimal. Therefore, according to the present study, the efficacy of octreotide administration is very low.
研究奥曲肽对急性实验性胰腺炎的影响。
通过向70只雄性Wistar大鼠的胰管逆行注射5%牛磺胆酸钠诱导急性胰腺炎。从胰腺炎诱导时（第1组和第2组）或12小时后（第3组和第4组）开始，每6小时皮下注射奥曲肽（50微克 = 0.5毫升）或等渗盐水，共24小时。在最后一剂奥曲肽或盐水注射6小时后，处死大鼠。另外10只大鼠用于基础生化测定。为分析存活率，又纳入10只大鼠。检测存活率、血清淀粉酶、脂肪酶和乳酸脱氢酶（LDH）浓度以及形态学研究（胰腺总面积和坏死百分比）。统计分析采用学生t检验、方差分析和Mantel-Haenszel检验。
除诱导时接受奥曲肽治疗的组中LDH水平略有升高外，各组在存活率、形态学分析或生化测定方面无显著差异。
奥曲肽给药对实验性重症急性胰腺炎的死亡率几乎没有影响。生化和形态学变化极小。因此，根据本研究，奥曲肽给药的疗效非常低。
