Misago N, Toda S, Sugihara H, Kohda H, Narisawa Y
Department of Internal Medicine, Saga Medical School, Japan.
Br J Dermatol. 1998 Jul;139(1):40-8. doi: 10.1046/j.1365-2133.1998.02312.x.
Using rat skin, we studied the influence of fat cells on the proliferation and differentiation of organoid hair follicle cells in a three-dimensional collagen gel matrix culture system. We cultured organoid hair follicles embedded in collagen gel under each of the following three conditions: cell-free collagen gel for control experiments (condition 1); co-culture with fat cells in close apposition (condition 2); and co-culture with fat cells in spatial separation (condition 3). Outgrowths of epithelial cells from the organoid hair follicles associated with perifollicular proliferation of fibroblasts were observed under conditions 1 and 3. Under condition 2, proliferation of both organoid hair follicle cells and fibroblasts was inhibited, but differentiation of the hair follicle cells appeared to be accelerated. Fat cells are considered to have an inhibitory effect on the proliferation of perifollicular fibroblasts, which might have resulted in the inhibition of hair follicle cell proliferation and also in the better maintenance of normal follicular structure and integrity, allowing for hair-type differentiation to proceed. A direct accelerating effect of fat cells on hair follicle differentiation may also have been responsible. In a physiological state (co-culture with keratinocytes on the collagen gel), similar results were observed under conditions 1 and 2. The different findings under conditions 2 and 3 may be due to either of two possibilities: either the concentration gradient of the soluble factors released from fat cells, acting on either the hair follicle cells or the perifollicular fibroblasts as an inhibitor of proliferation, caused the difference in the results, or direct contact between the organoid hair follicle cells and fat cells may have influenced the accelerating effect of fat cells on the differentiation of hair follicle cells.
我们使用大鼠皮肤，在三维胶原凝胶基质培养系统中研究了脂肪细胞对类器官毛囊细胞增殖和分化的影响。我们在以下三种条件下培养包埋于胶原凝胶中的类器官毛囊：无细胞胶原凝胶用于对照实验（条件1）；与脂肪细胞紧密并置共培养（条件2）；与脂肪细胞空间分离共培养（条件3）。在条件1和3下观察到类器官毛囊的上皮细胞生长，并伴有毛囊周围成纤维细胞的增殖。在条件2下，类器官毛囊细胞和成纤维细胞的增殖均受到抑制，但毛囊细胞的分化似乎加速。脂肪细胞被认为对毛囊周围成纤维细胞的增殖具有抑制作用，这可能导致毛囊细胞增殖受到抑制，同时也更好地维持了正常毛囊结构和完整性，从而使毛发类型分化得以进行。脂肪细胞对毛囊分化的直接促进作用也可能起到了作用。在生理状态下（在胶原凝胶上与角质形成细胞共培养），在条件1和2下观察到了类似的结果。条件2和3下的不同结果可能归因于两种可能性之一：要么是脂肪细胞释放的可溶性因子浓度梯度作为增殖抑制剂作用于毛囊细胞或毛囊周围成纤维细胞，导致了结果的差异，要么是类器官毛囊细胞与脂肪细胞之间的直接接触可能影响了脂肪细胞对毛囊细胞分化的促进作用。
