Falck B, Jacobsson S, Lindvall O, Nietsche U B
Scand J Plast Reconstr Surg. 1976;10(3):185-8. doi: 10.3109/02844317609012967.
Previous studies have demonstrated a specific cytoplasmic fluorescence in human melanocytes, as well as in pigmented nevi and in malignant melanomas, when the formaldehyde histofluorescence method for visualization of certain catechol and indole derivatives was used. In malignant melanoma two fluorogenic substances, dopa and cysteinyldopa, were found previously. In human melanocytes and benign nevi cells the fluorogenic catechols have so far not been characterized, since chemical analyses are difficult to perform on skin, due to the small amounts of catechols present. However, using split thickness skin quantitative determinations are possible by sensitive fluorometric methods. The chemical analyses of cysteinyldopa showed that in human adult skin most or all was located in the superficial layers. The only specific fluorescence in the thin skin was found in dendritic melanocytes. The findings leave little doubt that cysteinyldopa is stored in melanocytes although the possibility of a concomitant occurrence of other thioethers is not excluded. Nevi and giant nevi were also similarly studied and we found considerable amounts of cysteinyldopa in the nevi. It seems as if the cysteinyldopa is stored in the fluorescent nevi cells. There was no consistent difference in the content of the catechol derivatives between intradermal and compound nevi.
以往的研究表明，当使用甲醛组织荧光法来观察某些儿茶酚和吲哚衍生物时，在人类黑素细胞、色素痣及恶性黑素瘤中会出现特定的细胞质荧光。此前在恶性黑素瘤中发现了两种荧光物质，即多巴和半胱氨酰多巴。在人类黑素细胞和良性痣细胞中，由于皮肤中儿茶酚含量极少，难以进行化学分析，因此迄今为止尚未对荧光儿茶酚进行鉴定。然而，采用薄皮片时，通过灵敏的荧光测定法可以进行定量测定。对半胱氨酰多巴的化学分析表明在成人皮肤中，大部分或所有半胱氨酰多巴都位于表层。在薄皮肤中，唯一的特异性荧光见于树突状黑素细胞。这些发现无疑表明半胱氨酰多巴储存于黑素细胞中，尽管不排除同时存在其他硫醚的可能性。对痣和巨大型痣也进行了类似研究，我们在痣中发现了大量半胱氨酰多巴。似乎半胱氨酰多巴储存于荧光痣细胞中。皮内痣和混合痣中儿茶酚衍生物的含量没有一致的差异。
