Nickel W, Weber T, McNew J A, Parlati F, Söllner T H, Rothman J E
Cellular Biochemistry Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12571-6. doi: 10.1073/pnas.96.22.12571.
Membrane bilayer fusion has been shown to be mediated by v- and t-SNAREs initially present in separate populations of liposomes and to occur with high efficiency at a physiologically meaningful rate. Lipid mixing was demonstrated to involve both the inner and the outer leaflets of the membrane bilayer. Here, we use a fusion assay that relies on duplex formation of oligonucleotides introduced in separate liposome populations and report that SNARE proteins suffice to mediate complete membrane fusion accompanied by mixing of luminal content. We also find that SNARE-mediated membrane fusion does not compromise the integrity of liposomes.
膜双层融合已被证明是由最初存在于不同脂质体群体中的v-SNARE和t-SNARE介导的,并且以生理意义上有意义的速率高效发生。脂质混合被证明涉及膜双层的内层和外层小叶。在这里,我们使用一种融合测定法,该方法依赖于引入不同脂质体群体中的寡核苷酸的双链形成,并报告SNARE蛋白足以介导完全的膜融合并伴有腔内内容物的混合。我们还发现SNARE介导的膜融合不会损害脂质体的完整性。