由N-乙基马来酰亚胺敏感因子（NSF）、可溶性NSF附着蛋白（SNAPs）和SNARE复合体组成的20 S颗粒中亚基的排列
Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes.
作者信息
Hohl T M, Parlati F, Wimmer C, Rothman J E, Söllner T H, Engelhardt H
机构信息
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
出版信息
Mol Cell. 1998 Nov;2(5):539-48. doi: 10.1016/s1097-2765(00)80153-7.
Abstract
The structure of 20 S particles, consisting of NSF, SNAPs, and SNARE complexes, was analyzed by electron microscopy and fluorescence resonance energy transfer. Structural changes associated with the binding of alpha-SNAP and NSF to SNARE complexes define the contribution of each component to the 20 S particle structure. The synaptic SNARE complex forms a 2.5 x 15 nm rod. alpha-SNAP binds laterally to the rod, increasing its width but not its length. NSF binds to one end of the SNAP/SNARE complex; the resulting 20 S particles measure 22 nm in length and vary in width from 6 nm at their narrowest point to 13.5 nm at their widest. The transmembrane domains of VAMP and syntaxin emerge together at the NSF-distal end of 20 S particles, adjacent to the amino terminus of alpha-SNAP.
摘要
由N-乙基马来酰亚胺敏感因子（NSF）、可溶性NSF附着蛋白（SNAPs）和SNARE复合体组成的20S颗粒结构，通过电子显微镜和荧光共振能量转移进行了分析。与α-SNAP和NSF与SNARE复合体结合相关的结构变化，确定了每个组分对20S颗粒结构的贡献。突触SNARE复合体形成一个2.5×15纳米的杆状结构。α-SNAP横向结合到杆状结构上，增加其宽度但不增加长度。NSF结合到SNAP/SNARE复合体的一端；形成的20S颗粒长度为22纳米，宽度在最窄处为6纳米，最宽处为13.5纳米。囊泡相关膜蛋白（VAMP）和突触融合蛋白的跨膜结构域在20S颗粒的NSF远端共同出现，与α-SNAP的氨基末端相邻。
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本文引用的文献
1
Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP.与ATP复合的N-乙基马来酰亚胺敏感因子的ATP依赖性寡聚化结构域的结构
Nat Struct Biol. 1998 Sep;5(9):803-11. doi: 10.1038/1843.
2
The synaptic SNARE complex is a parallel four-stranded helical bundle.突触SNARE复合体是一个平行的四链螺旋束。
Nat Struct Biol. 1998 Sep;5(9):765-9. doi: 10.1038/1799.
3
Interaction of the N-ethylmaleimide-sensitive factor with AMPA receptors.N-乙基马来酰亚胺敏感因子与AMPA受体的相互作用。
Neuron. 1998 Aug;21(2):393-400. doi: 10.1016/s0896-6273(00)80548-6.
4
Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein.N-乙基马来酰亚胺敏感融合蛋白六聚化结构域的晶体结构
Cell. 1998 Aug 21;94(4):525-36. doi: 10.1016/s0092-8674(00)81593-7.
5
The AMPA receptor GluR2 C terminus can mediate a reversible, ATP-dependent interaction with NSF and alpha- and beta-SNAPs.AMPA受体GluR2的C末端可介导与NSF以及α-和β-SNAPs的可逆性、ATP依赖性相互作用。
Neuron. 1998 Jul;21(1):99-110. doi: 10.1016/s0896-6273(00)80518-8.
6
NSF binding to GluR2 regulates synaptic transmission.NSF与GluR2的结合调节突触传递。
Neuron. 1998 Jul;21(1):87-97. doi: 10.1016/s0896-6273(00)80517-6.
7
Functional reconstitution of ypt7p GTPase and a purified vacuole SNARE complex.Ypt7p GTP酶与纯化的液泡SNARE复合体的功能重建。
Science. 1998 Jul 31;281(5377):700-2. doi: 10.1126/science.281.5377.700.
8
Identification of a minimal core of the synaptic SNARE complex sufficient for reversible assembly and disassembly.鉴定出突触SNARE复合体的一个最小核心，其足以进行可逆组装和解聚。
Biochemistry. 1998 Jul 21;37(29):10354-62. doi: 10.1021/bi980542h.
9
A revised model for the oligomeric state of the N-ethylmaleimide-sensitive fusion protein, NSF.N-乙基马来酰亚胺敏感融合蛋白（NSF）寡聚状态的修正模型。
J Biol Chem. 1998 Jun 19;273(25):15675-81. doi: 10.1074/jbc.273.25.15675.
10
Protease resistance of syntaxin.SNAP-25.VAMP complexes. Implications for assembly and structure.syntaxin.SNAP-25.VAMP复合物的蛋白酶抗性。对组装和结构的影响。
J Biol Chem. 1998 May 1;273(18):11370-7. doi: 10.1074/jbc.273.18.11370.
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