关于四膜虫大核DNA的粘弹性研究。
Viscoelastic studies on Tetrahymena macronuclear DNA.
作者信息
Williams J B, Fleck E W, Hellier L E, Uhlenhopp E
出版信息
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5062-5. doi: 10.1073/pnas.75.10.5062.
Abstract
We have used viscoelastometry in an attempt to understand the physical organization of genetic material in Tetrahymena nuclei. The micronucleus or germ line nucleus is diploid. It divides mitotically during vegetative growth, and five pairs of chromosomes are seen in meiosis. The macronucleus, or somatic nucleus, is approximately 45-ploid, divides amitotically, and has no visible chromosomes at any stage. Viscoelastic analysis of Tetrahymena macronuclei reveals DNA Molecules of 2-3 X 10(10) daltons accounting for much, if not all, of the macronuclear DNA. Since the average chromosome in the micronucleus contains 2.4-2.7 X 10(10) daltons of DNA, we deduce that the macronucleus of Tetrahymena contains chromosome-sized DNA molecules.
摘要
我们利用粘弹性测定法来试图了解四膜虫细胞核中遗传物质的物理组织。微核或种系核是二倍体。它在营养生长期间进行有丝分裂，在减数分裂中可看到五对染色体。大核或体细胞核大约是45倍体，进行无丝分裂，在任何阶段都没有可见的染色体。对四膜虫大核的粘弹性分析表明，2 - 3×10¹⁰道尔顿的DNA分子占了大核DNA的大部分（如果不是全部的话）。由于微核中的平均染色体含有2.4 - 2.7×10¹⁰道尔顿的DNA，我们推断四膜虫的大核含有染色体大小的DNA分子。
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