Ueda T, Sakagami H, Abe K, Oishi I, Maruo A, Kondo H, Terashima T, Ichihashi M, Yamamura H, Minami Y
Department of Biochemistry, Kobe University, School of Medicine, Japan.
J Neurochem. 1999 Nov;73(5):2119-29.
Ca2+/calmodulin-dependent protein kinases (CaMKs) are believed to play important roles in the development and function of the nervous system. We report here the identification and expression of mouse CaMKIbeta (mCaMKIbeta), in particular mCaMKIbeta2, an isoform of mCaMKIbeta. During embryogenesis, the mCaMKIbeta2 gene is expressed mainly in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS, the expression of mCaMKIbeta2 is detected in the mantle zone, but not in the ventricular zone, suggesting its possible involvement in the differentiation of neurons. In the adult brain, mCaMKIbeta2 transcripts are detected at high levels in the anterior olfactory nuclei, piriform cortex, septal nuclei, bed nuclei of the stria terminalis, hippocampal pyramidal cells, dentate granule cells, amygdala, hypothalamic nuclei, parabrachial nucleus, and nucleus of the solitary tract. The distinct gene expression pattern suggests that mCaMKIbeta2 may also be involved in different mature neuronal functions from other CaMKs. In addition, mCaMKI/beta2 proteins are localized to the cytoplasm and nuclei, but not to nucleoli, suggesting that mCaMKIbeta2 proteins might be involved in the cytoplasmic and nuclear signal transduction of the nervous system.
钙离子/钙调蛋白依赖性蛋白激酶（CaMKs）被认为在神经系统的发育和功能中发挥重要作用。我们在此报告小鼠CaMKIβ（mCaMKIβ），特别是mCaMKIβ的一种亚型mCaMKIβ2的鉴定和表达。在胚胎发生过程中，mCaMKIβ2基因主要在神经系统中表达，包括脑、脊髓、三叉神经节和视网膜。在中枢神经系统内，mCaMKIβ2的表达在套层区被检测到，但在脑室区未被检测到，这表明它可能参与神经元的分化。在成年大脑中，mCaMKIβ2转录本在前嗅核、梨状皮质、隔核、终纹床核、海马锥体细胞、齿状颗粒细胞、杏仁核、下丘脑核、臂旁核和孤束核中高水平表达。这种独特的基因表达模式表明，mCaMKIβ2可能也参与与其他CaMKs不同的成熟神经元功能。此外，mCaMKI/β2蛋白定位于细胞质和细胞核，但不定位于核仁，这表明mCaMKIβ2蛋白可能参与神经系统的细胞质和核信号转导。
