Davidson NC, Clarke AR
Molecular Physics and Instrumentation Group, Department of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, U.K.
J Microsc. 1999 Nov;196(# (Pt 2)):266-72. doi: 10.1046/j.1365-2818.1999.00633.x.
An automatic image analysis system has been developed, that is capable of scanning a large sample area (70 mm x 70 mm) using transmitted light microscopy. The analysis, based on transparent algorithms, successfully discriminates between overlapping and touching fibres, so that sample preparation time is minimized. The system utilizes overlapping image frames to reconstruct partial fibre images and impart unique fibre length information in the range micrometres to millimetres. The software has been made adaptable to enable the accurate measurement of fibre lengths for fibres that curve over many millimetres. Data are presented illustrating the accurate measurement of fibre lengths over a large range of fibre aspect ratios.
已经开发出一种自动图像分析系统，该系统能够使用透射光显微镜扫描大面积样本区域（70毫米×70毫米）。基于透明算法的分析成功地区分了重叠和接触的纤维，从而将样本制备时间降至最低。该系统利用重叠图像帧来重建部分纤维图像，并赋予微米到毫米范围内独特的纤维长度信息。该软件已经进行了优化，能够精确测量弯曲数毫米的纤维的长度。文中给出的数据表明，该系统能够在很大的纤维长径比范围内精确测量纤维长度。
