通过傅里叶空间图像分析对纤维聚合进行定量分析。
Quantification of fibre polymerization through Fourier space image analysis.
作者信息
Nekouzadeh Ali, Genin Guy M
机构信息
Department of Biomedical Engineering , Washington University in Saint Louis , Saint Louis, MO, USA.
Department of Mechanical, Aerospace and Structural Engineering , Washington University in Saint Louis , Saint Louis, MO, USA ; Center for Materials Innovation , Washington University in Saint Louis , Saint Louis, MO, USA.
出版信息
Proc Math Phys Eng Sci. 2011 Aug 8;467(2132):2310-2329. doi: 10.1098/rspa.2010.0623. Epub 2011 Mar 9.
Abstract
Quantification of changes in the total length of randomly oriented and possibly curved lines appearing in an image is a necessity in a wide variety of biological applications. Here, we present an automated approach based upon Fourier space analysis. Scaled, band-pass filtered power spectral densities of greyscale images are integrated to provide a quantitative measurement of the total length of lines of a particular range of thicknesses appearing in an image. A procedure is presented to correct for changes in image intensity. The method is most accurate for two-dimensional processes with fibres that do not occlude one another.
摘要
在众多生物学应用中，对图像中出现的随机取向且可能弯曲的线条总长度变化进行量化是必要的。在此，我们提出一种基于傅里叶空间分析的自动化方法。对灰度图像的缩放、带通滤波功率谱密度进行积分，以提供对图像中出现的特定厚度范围线条总长度的定量测量。提出了一种校正图像强度变化的程序。该方法对于纤维不相互遮挡的二维过程最为准确。
相似文献
1
Quantification of fibre polymerization through Fourier space image analysis.通过傅里叶空间图像分析对纤维聚合进行定量分析。
Proc Math Phys Eng Sci. 2011 Aug 8;467(2132):2310-2329. doi: 10.1098/rspa.2010.0623. Epub 2011 Mar 9.
2
Extending the dynamic range of fibre length and fibre aspect ratios by automated image analysis.通过自动图像分析扩展纤维长度和纤维长径比的动态范围。
J Microsc. 1999 Nov;196(# (Pt 2)):266-72. doi: 10.1046/j.1365-2818.1999.00633.x.
3
Noise reduction and quantification of fiber orientations in greyscale images.灰度图像中纤维方向的降噪和量化。
PLoS One. 2020 Jan 16;15(1):e0227534. doi: 10.1371/journal.pone.0227534. eCollection 2020.
4
Quantitative assessment of collagen fibre orientations from two-dimensional images of soft biological tissues.从软生物组织的二维图像中定量评估胶原纤维方向。
J R Soc Interface. 2012 Nov 7;9(76):3081-93. doi: 10.1098/rsif.2012.0339. Epub 2012 Jul 4.
5
A method for estimating the fibre length in fibre-PLA composites.一种估算纤维-PLA 复合材料中纤维长度的方法。
J Microsc. 2013 Apr;250(1):15-20. doi: 10.1111/jmi.12012. Epub 2013 Jan 22.
6
On the orientational analysis of planar fibre systems from digital images.关于从数字图像对平面纤维系统进行取向分析
J Microsc. 2002 Jul;207(Pt 1):69-77. doi: 10.1046/j.1365-2818.2002.01041.x.
7
Orientational analysis of planar fibre systems observed as a Poisson shot-noise process.
J Microsc. 2007 Oct;228(Pt 1):88-96. doi: 10.1111/j.1365-2818.2007.01826.x.
8
Automatic diameter and orientation distribution determination of fibrous materials in micro X-ray CT imaging data.自动确定微 X 射线 CT 成像数据中纤维材料的直径和方向分布。
J Microsc. 2018 Dec;272(3):180-195. doi: 10.1111/jmi.12719. Epub 2018 Jun 6.
9
Perceptually accurate display of two greyscale images as a single colour image.将两幅灰度图像以单幅彩色图像的形式进行感知上准确的显示。
J Microsc. 2017 Oct;268(1):73-83. doi: 10.1111/jmi.12588. Epub 2017 May 30.
10
Automated nerve fibre size and myelin sheath measurement using microcomputer-based digital image analysis: theory, method and results.使用基于微型计算机的数字图像分析进行神经纤维大小和髓鞘测量：理论、方法与结果
J Neurosci Methods. 1994 Mar;51(2):229-38. doi: 10.1016/0165-0270(94)90015-9.
引用本文的文献
1
Energy dissipation in quasi-linear viscoelastic tissues, cells, and extracellular matrix.准线性黏弹性组织、细胞和细胞外基质中的能量耗散。
J Mech Behav Biomed Mater. 2018 Aug;84:198-207. doi: 10.1016/j.jmbbm.2018.05.011. Epub 2018 May 26.
2
Tissue constructs: platforms for basic research and drug discovery.组织工程构建体：基础研究与药物发现的平台。
Interface Focus. 2016 Feb 6;6(1):20150095. doi: 10.1098/rsfs.2015.0095.
3
Acetyl-L-Carnitine Prevents Methamphetamine-Induced Structural Damage on Endothelial Cells via ILK-Related MMP-9 Activity.乙酰左旋肉碱通过整合素连接激酶相关的基质金属蛋白酶 9 活性预防甲基苯丙胺诱导的内皮细胞结构损伤。
Mol Neurobiol. 2016 Jan;53(1):408-422. doi: 10.1007/s12035-014-8973-5. Epub 2014 Dec 3.
4
The role of mechanics in actin stress fiber kinetics.力学在肌动蛋白应力纤维动力学中的作用。
Exp Cell Res. 2013 Oct 1;319(16):2490-500. doi: 10.1016/j.yexcr.2013.06.017. Epub 2013 Jul 29.
5
Physically-induced cytoskeleton remodeling of cells in three-dimensional culture.三维培养中的细胞物理诱导的细胞骨架重构。
PLoS One. 2012;7(12):e45512. doi: 10.1371/journal.pone.0045512. Epub 2012 Dec 27.
本文引用的文献
1
Actin dynamics: from nanoscale to microscale.肌动蛋白动力学：从纳米尺度到微米尺度。
Annu Rev Biophys. 2010;39:91-110. doi: 10.1146/annurev.biophys.093008.131207.
2
Simulation of the contractile response of cells on an array of micro-posts.微柱阵列上细胞收缩反应的模拟。
Philos Trans A Math Phys Eng Sci. 2009 Sep 13;367(1902):3477-97. doi: 10.1098/rsta.2009.0097.
3
Mechanosensing in actin stress fibers revealed by a close correlation between force and protein localization.肌动蛋白应力纤维中的机械传感通过力与蛋白质定位之间的紧密相关性得以揭示。
J Cell Sci. 2009 May 15;122(Pt 10):1665-79. doi: 10.1242/jcs.042986. Epub 2009 Apr 28.
4
Stretch-activated force shedding, force recovery, and cytoskeletal remodeling in contractile fibroblasts.收缩性成纤维细胞中的牵张激活的力释放、力恢复和细胞骨架重塑。
J Biomech. 2008 Oct 20;41(14):2964-71. doi: 10.1016/j.jbiomech.2008.07.033. Epub 2008 Sep 20.
5
New insights into mechanism and regulation of actin capping protein.肌动蛋白封端蛋白的机制与调控新见解
Int Rev Cell Mol Biol. 2008;267:183-206. doi: 10.1016/S1937-6448(08)00604-7.
6
Dynamics of cellular focal adhesions on deformable substrates: consequences for cell force microscopy.可变形基质上细胞粘着斑的动力学：对细胞力显微镜的影响
Biophys J. 2008 Jul;95(2):527-39. doi: 10.1529/biophysj.107.127399. Epub 2008 Apr 11.
7
Comparison of 2D fiber network orientation measurement methods.二维纤维网络取向测量方法的比较
J Biomed Mater Res A. 2009 Feb;88(2):322-31. doi: 10.1002/jbm.a.31847.
8
A simplified approach to quasi-linear viscoelastic modeling.准线性粘弹性建模的一种简化方法。
J Biomech. 2007;40(14):3070-8. doi: 10.1016/j.jbiomech.2007.03.019. Epub 2007 May 17.
9
Accurate centerline detection and line width estimation of thick lines using the radon transform.利用拉东变换对粗线进行精确的中心线检测和线宽估计。
IEEE Trans Image Process. 2007 Feb;16(2):310-6. doi: 10.1109/tip.2006.887731.
10
Structure/function analysis of the interaction of phosphatidylinositol 4,5-bisphosphate with actin-capping protein: implications for how capping protein binds the actin filament.磷脂酰肌醇4,5-二磷酸与肌动蛋白封端蛋白相互作用的结构/功能分析：对封端蛋白如何结合肌动蛋白丝的启示
J Biol Chem. 2007 Feb 23;282(8):5871-9. doi: 10.1074/jbc.M609850200. Epub 2006 Dec 19.
Zotero 插件