Diethelm A G, Chambers L, Sachs G, Balch C M, Phillips S J, Thiry C
Surgery. 1979 Feb;85(2):159-65.
Human thymocytes separated by a Ficoll gradient produced a cell population that was 99% pure thymocytes and free of platelets, leukocytes, and epithelial cells. These cells, disrupted by a nitrogen bomb, produced a membrane-ribosome antigen fraction confirmed by enzyme analysis. Equine antithymocyte membrane-immunoglobulin G (ATM-IgC) prepared against this antigen in four of five horses contained immunosuppressive properties capable of prolonging monkey skin allograft survival longer than 21 days. No adverse effects were noted by the intramuscular and intravenous administration of this antisera to primates, and autopsy examination showed marked depletion of paracortical lymphocytes in the spleen and mesenteric lymph nodes. A moderate thrombocytopenia occurred during a 4 hour intravenous administration of ATM-IgG to primates with a marked decrease in the peripheral lymphocyte count. The deposition of ATM-IgG upon monkey glomerular basement membrane could not be demonstrated by immunofluorescent techniques. The specificity of this globulin to contain anti-T-cell antibody was confirmed by an immunofluorescent assay in that ATM-IgG reacted with both human thymocytes and peripheral blood thymus-dependent cells, but was nonreactive when tested against a panel of human cells free of thymus-dependent antigens.
通过菲可梯度分离的人胸腺细胞产生了一个细胞群体，该群体由99%的纯胸腺细胞组成，且不含血小板、白细胞和上皮细胞。这些细胞经氮弹破碎后，产生了经酶分析证实的膜核糖体抗原组分。在五匹马中的四匹马中，针对该抗原制备的马抗胸腺细胞膜免疫球蛋白G（ATM-IgG）具有免疫抑制特性，能够使猴皮肤同种异体移植存活时间延长超过21天。对灵长类动物进行肌肉注射和静脉注射这种抗血清未观察到不良反应，尸检显示脾脏和肠系膜淋巴结的副皮质淋巴细胞明显减少。在对灵长类动物静脉注射ATM-IgG的4小时过程中出现了中度血小板减少症，外周淋巴细胞计数显著下降。免疫荧光技术未能证实ATM-IgG在猴肾小球基底膜上的沉积。通过免疫荧光测定证实了这种球蛋白含有抗T细胞抗体的特异性，因为ATM-IgG与人胸腺细胞和外周血胸腺依赖细胞均发生反应，但在针对一组不含胸腺依赖抗原的人类细胞进行测试时无反应。
