Lee S H, Park Y B, Bae K H, Bok S H, Kwon Y K, Lee E S, Choi M S
Department of Nutrition and Food Science, Kyungpook National University, Taegu, South Korea.
Ann Nutr Metab. 1999;43(3):173-80. doi: 10.1159/000012783.
The effects of dietary supplementation of a citrus bioflavonoid, naringenin, on the cholesterol metabolism were studied. For 42 days male rats were fed a 1% (wt/wt) high-cholesterol diet with or without a naringenin supplementation (0.1%, wt/wt) to study its effect on plasma lipid levels, hepatic lipid contents, activities of hepatic acyl coenzyme A:cholesterol O-acyltransferase (ACAT) and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, and the excretion of fecal neutral sterols. Naringenin did not significantly alter the concentration of plasma triglycerides, but lowered the plasma cholesterol (3.80 vs. 3.12 mmol/l) concentration and the hepatic cholesterol content (70.3 vs. 54.0 mg/g) significantly (p < 0.05) compared to those of the controls. HMG-CoA reductase (1,879.0 vs. 1,715.0 pmol/min/mg) and ACAT activities (806.0 vs. 563.0 pmol/min/mg) were significantly lower in the naringenin-supplemented group than in controls. Naringenin supplementation caused a marked decrease in the excretion of fecal neutral sterols (242.9 mg/day) compared to the controls (521.9 mg/day). These results show that naringenin lowers the plasma and hepatic cholesterol concentrations by suppressing HMG-CoA reductase and ACAT in rats fed a high-cholesterol diet.
研究了膳食补充柑橘生物类黄酮柚皮素对胆固醇代谢的影响。在42天的时间里，给雄性大鼠喂食含1%（重量/重量）高胆固醇的饮食，其中一组添加柚皮素（0.1%，重量/重量），另一组不添加，以研究其对血浆脂质水平、肝脏脂质含量、肝脏酰基辅酶A：胆固醇O-酰基转移酶（ACAT）和3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶活性以及粪便中性固醇排泄的影响。柚皮素对血浆甘油三酯浓度没有显著影响，但与对照组相比，显著降低了血浆胆固醇浓度（3.80对3.12 mmol/l）和肝脏胆固醇含量（70.3对54.0 mg/g）（p<0.05）。补充柚皮素组的HMG-CoA还原酶活性（1,879.0对1,715.0 pmol/min/mg）和ACAT活性（806.0对563.0 pmol/min/mg）显著低于对照组。与对照组（521.9 mg/天）相比，补充柚皮素导致粪便中性固醇排泄显著减少（242.9 mg/天）。这些结果表明，在喂食高胆固醇饮食的大鼠中，柚皮素通过抑制HMG-CoA还原酶和ACAT来降低血浆和肝脏胆固醇浓度。
