RNA引发的DNA合成：HeLa细胞DNA聚合酶α的特异性催化作用
RNA-primed DNA synthesis: specific catalysis by HeLa cell DNA polymerase alpha.
作者信息
Spadari S, Weissbach A
出版信息
Proc Natl Acad Sci U S A. 1975 Feb;72(2):503-7. doi: 10.1073/pnas.72.2.503.
Abstract
We have analyzed and compared the responses of the three major HeLa cell DNA polymerases (alpha, beta, and gamma) to a HeLa DNA template with short RNA or DNA primers hybridized to it. Only DNA polymerase alpha is able to synthesize DNA covalently bonded to the RNA primer via a 3' yields 5' phosphodiester bond. 32P transfer experiments showed that all combinations of ribo- and deoxyribonucleotides are represented in the RNA-DNA linkages but their distribution is nonrandom. The RNA-DNA linked molecules base-paired to a HeLa DNA template strand represent a possible "natural" in vitro primer-template for DNA polymerases and can be extended by all three DNA polymerases (alpha, beta, and gamma). These findings indicate that DNA polymerases beta and gamma are capable of DNA-primed but not RNA-PRIMED DNA synthesis, while DNA polymerase alpha is capable of both RNA-primed and DAN-primed DNA synthesis.
摘要
我们分析并比较了三种主要的海拉细胞DNA聚合酶（α、β和γ）对与短RNA或DNA引物杂交的海拉细胞DNA模板的反应。只有DNA聚合酶α能够通过3'生成5'磷酸二酯键合成与RNA引物共价结合的DNA。32P转移实验表明，核糖核苷酸和脱氧核糖核苷酸的所有组合都存在于RNA-DNA连接中，但它们的分布并非随机。与海拉细胞DNA模板链碱基配对的RNA-DNA连接分子代表了一种可能的DNA聚合酶“天然”体外引物-模板，并且可以被所有三种DNA聚合酶（α、β和γ）延伸。这些发现表明，DNA聚合酶β和γ能够进行DNA引发的而非RNA引发的DNA合成，而DNA聚合酶α既能进行RNA引发的DNA合成，也能进行DNA引发的DNA合成。
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