一种来自人组织培养细胞的新型合成RNA依赖性DNA聚合酶（海拉细胞-成纤维细胞-合成寡核苷酸-模板-纯化酶）
A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes).
作者信息
Fridlender B, Fry M, Bolden A, Weissbach A
出版信息
Proc Natl Acad Sci U S A. 1972 Feb;69(2):452-5. doi: 10.1073/pnas.69.2.452.
Abstract
Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.
摘要
人类组织培养细胞中存在两种能够复制合成RNA聚合物的DNA聚合酶。这两种酶均已被纯化了约500倍，存在于源自宫颈癌的HeLa细胞以及源自人胚胎肺组织的正常二倍体细胞系WI-38细胞中。这些合成RNA依赖性DNA聚合酶可通过高效复制合成寡核苷酸-均聚物复合物的核糖链的能力来鉴定，在这方面与HeLa细胞中发现的已知DNA依赖性DNA聚合酶不同。这些新的DNA聚合酶的模板需求类似于RNA肿瘤病毒的RNA依赖性DNA聚合酶。
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