Bertazzoni U, Stefanini M, Pedrali-Noy G, Nuzzo F, Falaschi A
Nucleic Acids Res. 1977 Jan;4(1):141-8. doi: 10.1093/nar/4.1.141.
We have determined the levels of DNA-polymerases-alpha and-beta in fibroblasts obtained from normal subjects and from patients with Xeroderma Pigmentosum (XP) belonging to three different complementation groups and to the variant form. The assays have been performed in crude extracts and after fractionation on sucrose gradients. The levels of alpha and beta-polymerases in the different cases of XP were found to lie within the same range as the control values, and no correlation was found with the severity of the symptoms. The sedimentation coefficients of the two polymerases from all the pathological lines were identical to those of the normal fibroblasts.
我们已经测定了从正常受试者以及患有色素性干皮病（XP）的患者（属于三个不同互补组和变异型）获取的成纤维细胞中DNA聚合酶α和β的水平。检测在粗提物中进行，并在蔗糖梯度上分级分离后进行。发现不同XP病例中的α和β聚合酶水平与对照值处于同一范围内，并且未发现与症状严重程度相关。所有病理细胞系中这两种聚合酶的沉降系数与正常成纤维细胞的沉降系数相同。
