正常及着色性干皮病成纤维细胞中DNA聚合酶α和β的水平
Levels of DNA polymerase-alpha and beta in normal and xeroderma pigmentosum fibroblasts.
作者信息
Bertazzoni U, Stefanini M, Pedrali-Noy G, Nuzzo F, Falaschi A
出版信息
Nucleic Acids Res. 1977 Jan;4(1):141-8. doi: 10.1093/nar/4.1.141.
Abstract
We have determined the levels of DNA-polymerases-alpha and-beta in fibroblasts obtained from normal subjects and from patients with Xeroderma Pigmentosum (XP) belonging to three different complementation groups and to the variant form. The assays have been performed in crude extracts and after fractionation on sucrose gradients. The levels of alpha and beta-polymerases in the different cases of XP were found to lie within the same range as the control values, and no correlation was found with the severity of the symptoms. The sedimentation coefficients of the two polymerases from all the pathological lines were identical to those of the normal fibroblasts.
摘要
我们已经测定了从正常受试者以及患有色素性干皮病（XP）的患者（属于三个不同互补组和变异型）获取的成纤维细胞中DNA聚合酶α和β的水平。检测在粗提物中进行，并在蔗糖梯度上分级分离后进行。发现不同XP病例中的α和β聚合酶水平与对照值处于同一范围内，并且未发现与症状严重程度相关。所有病理细胞系中这两种聚合酶的沉降系数与正常成纤维细胞的沉降系数相同。
相似文献
1
Levels of DNA polymerase-alpha and beta in normal and xeroderma pigmentosum fibroblasts.正常及着色性干皮病成纤维细胞中DNA聚合酶α和β的水平
Nucleic Acids Res. 1977 Jan;4(1):141-8. doi: 10.1093/nar/4.1.141.
2
DNA polymerases alpha beta and gamma in inherited diseases affecting DNA repair.影响DNA修复的遗传性疾病中的DNA聚合酶α、β和γ
Nucleic Acids Res. 1978 Jun;5(6):2189-96. doi: 10.1093/nar/5.6.2189.
3
Levels of DNA polymerases alpha, beta, and gamma in control and repair-deficient human diploid fibroblasts 1.对照及修复缺陷型人二倍体成纤维细胞1中DNA聚合酶α、β和γ的水平
Nucleic Acids Res. 1977 Jun;4(6):2029-37. doi: 10.1093/nar/4.6.2029.
4
Xeroderma pigmentosum variant (XP-V) correcting protein from HeLa cells has a thymine dimer bypass DNA polymerase activity.来自人宫颈癌细胞系的着色性干皮病变异型（XP-V）校正蛋白具有胸腺嘧啶二聚体旁路DNA聚合酶活性。
EMBO J. 1999 Jun 15;18(12):3491-501. doi: 10.1093/emboj/18.12.3491.
5
Xeroderma pigmentosum variant and error-prone DNA polymerases.着色性干皮病变异型与易出错的DNA聚合酶。
Biochimie. 2003 Nov;85(11):1123-32. doi: 10.1016/j.biochi.2003.10.009.
6
Levels of some enzymes acting on DNA in xeroderma pigmentosum.着色性干皮病中一些作用于DNA的酶的水平。
Nucleic Acids Res. 1974 Feb;1(2):193-202. doi: 10.1093/nar/1.2.193.
7
An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts.A组和D组着色性干皮病成纤维细胞中脱嘌呤DNA内切酶活性改变。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1169-73. doi: 10.1073/pnas.73.4.1169.
8
Overproduction of DNA polymerase eta does not raise the spontaneous mutation rate in diploid human fibroblasts.DNA聚合酶η的过量产生不会提高二倍体人类成纤维细胞的自发突变率。
DNA Repair (Amst). 2005 Jun 8;4(6):714-24. doi: 10.1016/j.dnarep.2005.04.002.
9
The repair of identified large DNA adducts induced by 4-nitroquinoline-1-oxide in normal or xeroderma pigmentosum group A human fibroblasts, and the role of DNA polymerases alpha or delta.4-硝基喹啉-1-氧化物诱导的正常或A型着色性干皮病人类成纤维细胞中已识别的大DNA加合物的修复，以及DNA聚合酶α或δ的作用。
Carcinogenesis. 1989 Jul;10(7):1197-201. doi: 10.1093/carcin/10.7.1197.
10
Overexpression of matrix metalloproteinase 1 in dermal fibroblasts from DNA repair-deficient/cancer-prone xeroderma pigmentosum group C patients.DNA修复缺陷/易患癌症的着色性干皮病C组患者真皮成纤维细胞中基质金属蛋白酶1的过表达。
Oncogene. 2008 Sep 4;27(39):5223-32. doi: 10.1038/onc.2008.153. Epub 2008 May 12.
引用本文的文献
1
Isolation and characterization of monoclonal antibodies directed against the DNA repair enzyme uracil DNA glycosylase from human placenta.针对人胎盘DNA修复酶尿嘧啶DNA糖基化酶的单克隆抗体的分离与鉴定
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5822-6. doi: 10.1073/pnas.80.19.5822.
2
Levels of DNA polymerases alpha, beta, and gamma in control and repair-deficient human diploid fibroblasts 1.对照及修复缺陷型人二倍体成纤维细胞1中DNA聚合酶α、β和γ的水平
Nucleic Acids Res. 1977 Jun;4(6):2029-37. doi: 10.1093/nar/4.6.2029.
3
Differential susceptibilities of DNA polymerases-alpha and -beta to polyanions.DNA聚合酶α和β对聚阴离子的不同敏感性。
Nucleic Acids Res. 1978 Sep;5(9):3427-38. doi: 10.1093/nar/5.9.3427.
4
DNA polymerases alpha beta and gamma in inherited diseases affecting DNA repair.影响DNA修复的遗传性疾病中的DNA聚合酶α、β和γ
Nucleic Acids Res. 1978 Jun;5(6):2189-96. doi: 10.1093/nar/5.6.2189.
5
A cell-free assay measuring repair DNA synthesis in human fibroblasts.一种用于测量人成纤维细胞中修复性DNA合成的无细胞检测方法。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1887-91. doi: 10.1073/pnas.75.4.1887.
本文引用的文献
1
2
Defective repair replication of DNA in xeroderma pigmentosum.着色性干皮病中DNA修复复制缺陷。
Nature. 1968 May 18;218(5142):652-6. doi: 10.1038/218652a0.
3
Ultraviolet-stimulated thymidine incorporation in xeroderma pigmentosum lymphocytes.紫外线刺激的着色性干皮病淋巴细胞中的胸腺嘧啶核苷掺入
J Lab Clin Med. 1971 May;77(5):759-67.
4
Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA.着色性干皮病细胞在修复其DNA紫外线损伤时不执行第一步的证据。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035-41. doi: 10.1073/pnas.64.3.1035.
5
Xeroderma pigmentosum: a human disease in which an initial stage of DNA repair is defective.着色性干皮病：一种人类疾病，其DNA修复的初始阶段存在缺陷。
Proc Natl Acad Sci U S A. 1969 Jun;63(2):428-35. doi: 10.1073/pnas.63.2.428.
6
Xeroderma pigmentosum: variants with normal DNA repair and normal sensitivity to ultraviolet light.着色性干皮病：具有正常DNA修复能力且对紫外线敏感性正常的变异型。
J Invest Dermatol. 1972 Mar;58(3):124-8. doi: 10.1111/1523-1747.ep12538913.
7
Use of UV endonuclease from Micrococcus luteus to monitor the progress of DNA repair in UV-irradiated human cells.使用来自藤黄微球菌的紫外线核酸内切酶监测紫外线照射的人类细胞中DNA修复的进程。
Mutat Res. 1973 Aug;19(2):245-56. doi: 10.1016/0027-5107(73)90083-3.
8
Variation of deoxyribonucleic acid polymerase activities during rat liver regeneration.大鼠肝脏再生过程中脱氧核糖核酸聚合酶活性的变化
J Biol Chem. 1972 Dec 25;247(24):7948-50.
9
Repair enzyme suggested by mammalian endonuclease activity specific for ultraviolet-irradiated DNA.由对紫外线照射的DNA具有特异性的哺乳动物内切核酸酶活性所提示的修复酶。
Nat New Biol. 1972 Oct 11;239(93):172-3. doi: 10.1038/newbio239172a0.
10
Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization.通过体细胞杂交证明的着色性干皮病的遗传异质性。
Nat New Biol. 1972 Jul 19;238(81):80-3. doi: 10.1038/newbio238080a0.
Zotero 插件