D18S535、D1S1656和D10S2325：用于法医遗传学的三个高效短串联重复序列

D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics.

作者信息

Wiegand P, Lareu M V, Schürenkamp M, Kleiber M, Brinkmann B

机构信息

Institute of Legal Medicine, Martin-Luther-Universität Halle-Wittenberg, Franzosenweg 1, D-06112 Halle/Saale, Germany.

出版信息

Int J Legal Med. 1999;112(6):360-3. doi: 10.1007/pl00007705.

DOI:10.1007/pl00007705

PMID:10550594

Abstract

Three short tandem repeat (STR) polymorphisms characterized by PCR product length < 175 bp were investigated. D18S535 and D1S1656 contained a 4 bp unit as basic repeat motif, D10S2325 a 5 bp unit. The heterozygosity rates were 0.76 (D18S535), 0.88 (D10S2325) and 0. 90 (D1S1656), leading to a combined discrimination power of 0.9999. In contrast to D10S2325 and D18S535, which showed a homogeneous repeat array without any variation in the repeat motifs, repeat length and sequence variation was found for D1S1656. Robust typing results could be observed for all three STRs using highly degraded DNA.

摘要

研究了三种以PCR产物长度<175 bp为特征的短串联重复序列（STR）多态性。D18S535和D1S1656包含一个4 bp单元作为基本重复基序，D10S2325包含一个5 bp单元。杂合度分别为0.76（D18S535）、0.88（D10S2325）和0.90（D1S1656），组合鉴别力为0.9999。与D10S2325和D18S535不同，后者显示出均匀的重复阵列，重复基序没有任何变化，而D1S1656发现了重复长度和序列变异。使用高度降解的DNA对所有三个STR都能观察到可靠的分型结果。