西班牙东北部巴塞罗那地区八个Y染色体短串联重复序列（STR）位点的单倍型频率。

Haplotype frequencies of eight Y-chromosome STR loci in Barcelona (North-East Spain).

作者信息

Gené M, Borrego N, Xifró A, Piqué E, Moreno P, Huguet E

机构信息

Forensic Genetics Laboratory, Department of Legal Medicine, Faculty of Medicine, University of Barcelona, C. Casanova 143, E-08036 Barcelona, Spain.

出版信息

Int J Legal Med. 1999;112(6):403-5. doi: 10.1007/s004140050025.

DOI:10.1007/s004140050025

PMID:10550606

Abstract

Haplotype frequencies for eight Y-chromosomal short tandem repeat (STR) loci were determined in paragraph signa population sample from Barcelona (NE Spain). After PCR amplification and denaturing PAGE electrophoresis, DYS19, DYS388, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 loci were typed. Complete eight paragraph signY-chromosomal STRs haplotypes could be formed for 223 subjects, among which 137 different haplotypes were observed. The most common haplotype was shared by 13% of the sample, while 108 haplotypes were unique. The discrimination capacity was 61.5% and the gene diversity was 0.978. From the forensic point of view the combined polymorphisms provide a high diagnostic efficiency.

摘要

在来自巴塞罗那（西班牙东北部）的一组人群样本中，测定了8个Y染色体短串联重复序列（STR）位点的单倍型频率。经过聚合酶链反应（PCR）扩增和变性聚丙烯酰胺凝胶电泳后，对DYS19、DYS388、DYS389 I/II、DYS390、DYS391、DYS392和DYS393位点进行分型。223名受试者可形成完整的8个Y染色体STR单倍型，其中观察到137种不同的单倍型。最常见的单倍型在样本中占13%，而108种单倍型是独特的。鉴别能力为61.5%，基因多样性为0.978。从法医学角度来看，这些组合多态性具有很高的诊断效率。