甜菜夜蛾多粒包埋核型多角体病毒（AgMNPV）解旋酶基因的鉴定、表达及系统发育分析

Identification, expression and phylogenetic analysis of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus (AgMNPV) Helicase gene.

作者信息

de Lima Leoberto, Pinedo Francisco José Rivera, Ribeiro Bergmann Morais, Zanotto Paolo Marinho Andrade, Wolff José Luiz Caldas

机构信息

Laboratório de Virologia Molecular, Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Av. Dr. Cândido Xavier da Almeida Souza 200, SP, Brazil.

出版信息

Virus Genes. 2004 Dec;29(3):345-52. doi: 10.1007/s11262-004-7438-8.

DOI:10.1007/s11262-004-7438-8

PMID:15550775

Abstract

The helicase gene from Anticarsia gemmatalis multicapsid nucleopolyhedrovirus (AgMNPV) was identified and localized in the 58.85-65.90 m.u. of the viral genomic map. This gene encodes a putative polypeptide of 1221 amino acids containing motifs homologous to those found in the helicase superfamily. Expression of the AgMNPV helicase was observed as early as 4h post-infection (p.i.) up until 10 h p.i. A unique early transcription initiation site was observed upstream a putative TATA box. Phylogenetic analysis of the helicase genes of 23 baculoviruses indicated that the AgMNPV helicase is closely related to the helicase genes from Epiphyas postvitanna multicapsid nucleopolyhedrovirus and Choristoneura fumiferana defective nucleopolyhedrovirus.

摘要

已鉴定出来自豆银纹夜蛾多粒包埋核多角体病毒（AgMNPV）的解旋酶基因，并将其定位在病毒基因组图谱的58.85-65.90个图谱单位处。该基因编码一个推定的由1221个氨基酸组成的多肽，其中包含与解旋酶超家族中发现的基序同源的基序。早在感染后4小时（p.i.）直至感染后10小时都观察到AgMNPV解旋酶的表达。在一个推定的TATA框上游观察到一个独特的早期转录起始位点。对23种杆状病毒的解旋酶基因进行的系统发育分析表明，AgMNPV解旋酶与来自苹果蠹蛾多粒包埋核多角体病毒和云杉色卷蛾缺陷核多角体病毒的解旋酶基因密切相关。