Runova V F, Poliakova T M, Lodinova L M
Zh Mikrobiol Epidemiol Immunobiol. 1978 Aug(8):96-100.
Various methods for obtaining staphylococcus allergens were approbated. Biological activity of the preparation proved to depend on the method of obtaining it determining the chemical compostition of the preparation, and on the properties of the strain used. A method of alkaline extraction and a strain with marked pathogenicity signs (strain 209) can be recommended for the preparation of staphylococcus allergen. Biological activity of the allergen in case of its approbation in the same dose by protein on laboratory animals and patients proved to be the same.
对多种获取葡萄球菌过敏原的方法进行了验证。结果表明,制剂的生物活性取决于获取方法(该方法决定了制剂的化学组成)以及所用菌株的特性。对于制备葡萄球菌过敏原,可推荐采用碱性提取法以及具有明显致病性特征的菌株(209 株)。在实验室动物和患者身上以相同剂量的蛋白质对该过敏原进行验证时,其生物活性被证明是相同的。