Cohen S, Carpenter G
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1317-21. doi: 10.1073/pnas.72.4.1317.
A polypeptide hormone has been isolated from human urine, human epidermal growth factor. It was assayed by its ability to compete with 125I-labeled mouse-derived epidermal growth factor in binding to human foreskin fibroblasts. The biological effects of the human polypeptide are similar to those previously described for the mouse hormone. These include the stimulation of the growth in vitro of human foreskin fibroblasts and corneal epithelial cells in organ culture, and the in vivo induction of precocious eyelid opening in the newborn mouse. The amino acid compositions of the two polypeptides differ, although certain similarities are present. The estimated molecular weight of the human polypeptide, 5300-5500, is slightly lower than that of the mouse hormone. Both polypeptides apparently compete for the same site on the cell membrane; and antibodies to the mouse polypeptide crossreact to some extent with the human hormone.
一种多肽激素已从人尿中分离出来，即人表皮生长因子。通过其与125I标记的小鼠源表皮生长因子竞争结合人包皮成纤维细胞的能力来进行测定。该人源多肽的生物学效应与先前描述的小鼠激素的效应相似。这些效应包括刺激人包皮成纤维细胞和器官培养中的角膜上皮细胞的体外生长，以及在新生小鼠体内诱导早熟睁眼。尽管存在某些相似之处，但这两种多肽的氨基酸组成不同。人源多肽的估计分子量为5300 - 5500，略低于小鼠激素。两种多肽显然竞争细胞膜上的同一位点；并且针对小鼠多肽的抗体与人源激素有一定程度的交叉反应。
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