垂体提取物和类固醇激素对3T3细胞生长的控制
Pituitary extracts and steroid hormones in the control of 3T3 cell growth.
作者信息
Armelin H A
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6. doi: 10.1073/pnas.70.9.2702.
Abstract
Results are reported showing that pituitary extracts stimulate growth of 3T3 cells, an established line of mouse fibroblast. Cells were cultured in medium containing an organic buffer (HEPES; N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), which increases the serum requirement for cell growth. The pituitary growth-prompting activity is (a) not dialyzable; (b) thermolabile; (c) protease sensitive; and (d) enhanced by hydrocortisone (a steroid hormone). Activity was quantitatively assayed by measurement of stimulation of cell division or [(3)H]thymidine uptake into DNA. The active species is probably different from pituitary protein hormones and it seems to be effective at concentrations observed for protein hormones in vivo. 3T3 cells transformed by simian virus 40 are not responsive to this activity.
摘要
研究结果表明，垂体提取物能刺激3T3细胞（一种已建立的小鼠成纤维细胞系）的生长。细胞在含有有机缓冲剂（HEPES；N-2-羟乙基哌嗪-N'-2-乙磺酸）的培养基中培养，该缓冲剂会增加细胞生长对血清的需求。垂体的促生长活性具有以下特点：（a）不可透析；（b）对热不稳定；（c）对蛋白酶敏感；（d）氢化可的松（一种类固醇激素）可增强其活性。通过测量细胞分裂的刺激或[³H]胸苷掺入DNA来定量测定活性。活性物质可能与垂体蛋白激素不同，并且在体内观察到的蛋白激素浓度下似乎具有活性。被猿猴病毒40转化的3T3细胞对这种活性无反应。
相似文献
1
Pituitary extracts and steroid hormones in the control of 3T3 cell growth.垂体提取物和类固醇激素对3T3细胞生长的控制
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6. doi: 10.1073/pnas.70.9.2702.
2
Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.一种成纤维细胞生长因子的定位及其单独作用以及与氢化可的松共同作用对3T3细胞生长的影响。
Nature. 1974 May 10;249(453):123-7. doi: 10.1038/249123a0.
3
Role of growth factors and estrogen as modulators of growth, differentiation, and expression of gonadotropin subunit genes in primary cultured sheep pituitary cells.生长因子和雌激素作为原代培养绵羊垂体细胞中生长、分化及促性腺激素亚基基因表达调节因子的作用
Endocrinology. 1994 Feb;134(2):935-44. doi: 10.1210/endo.134.2.8299588.
4
Polyamine metabolism, RNA synthesis, and proliferation in density-Inhibited 3T3 cells.多胺代谢、RNA合成以及密度抑制的3T3细胞中的增殖
Arch Biochem Biophys. 1976 Feb;172(2):551-7. doi: 10.1016/0003-9861(76)90107-7.
5
Stimulation of division of sparse and confluent 3T3 cell populations by a fibroblast growth factor, dexamethasone, and insulin.成纤维细胞生长因子、地塞米松和胰岛素对稀疏及汇合状态的3T3细胞群体分裂的刺激作用。
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4584-8. doi: 10.1073/pnas.71.11.4584.
6
Stimulation of 2-deoxyglucose uptake in growth-inhibited BALB-C 3T3 and revertant SV40-transformed 3T3 cells.生长受抑制的BALB - C 3T3细胞和SV40转化的3T3回复细胞中2 - 脱氧葡萄糖摄取的刺激作用。
Exp Cell Res. 1974 Mar 15;84(1):335-50. doi: 10.1016/0014-4827(74)90413-3.
7
Steroid hormones mediate reversible phenotypic transition between transformed and untransformed states in mouse fibroblasts.类固醇激素介导小鼠成纤维细胞在转化状态和未转化状态之间的可逆表型转变。
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2805-9. doi: 10.1073/pnas.75.6.2805.
8
Growth control in cultured mouse fibroblasts: induction of the pleiotypic and mitogenic responses by a purified growth factor.培养的小鼠成纤维细胞中的生长控制：一种纯化生长因子诱导的多型性和促有丝分裂反应。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2600-4. doi: 10.1073/pnas.71.7.2600.
9
Induction of DNA synthesis and cell division in human diploid skin fibroblasts by fibroblast growth factor.成纤维细胞生长因子对人二倍体皮肤成纤维细胞DNA合成及细胞分裂的诱导作用。
Exp Cell Res. 1976 Jan;97:120-6. doi: 10.1016/0014-4827(76)90661-3.
10
Mouse brain extract with "fibroblast growth factor"-activity.具有“成纤维细胞生长因子”活性的小鼠脑提取物。
Exp Pathol (Jena). 1977;14(6):328-33. doi: 10.1016/s0014-4908(77)80052-5.
引用本文的文献
1
Whole-genome resequencing revealed genetic structure and specific breed identification loci of Ningxia Jingyuan chicken breed (Gallus gallus).全基因组重测序揭示了宁夏泾源鸡品种（家鸡）的遗传结构和特定品种鉴定位点。
BMC Genomics. 2025 Aug 25;26(1):772. doi: 10.1186/s12864-025-11927-w.
2
Fibroblast Growth Factors in Cardiovascular Disease.成纤维细胞生长因子在心血管疾病中的作用。
J Atheroscler Thromb. 2024 Nov 1;31(11):1496-1511. doi: 10.5551/jat.RV22025. Epub 2024 Aug 22.
3
Epidermal growth factor receptor-dependent stimulation of differentiation by human papillomavirus type 16 E5.人乳头瘤病毒 16 型 E5 通过表皮生长因子受体依赖性刺激分化。
Virology. 2024 Feb;590:109952. doi: 10.1016/j.virol.2023.109952. Epub 2023 Dec 12.
4
Liver organoid culture methods.肝脏类器官培养方法。
Cell Biosci. 2023 Nov 1;13(1):197. doi: 10.1186/s13578-023-01136-x.
5
Fibroblast growth factor 2.成纤维细胞生长因子 2。
Differentiation. 2024 Sep-Oct;139:100733. doi: 10.1016/j.diff.2023.10.001. Epub 2023 Oct 12.
6
Receptor Tyrosine Kinase: Still an Interesting Target to Inhibit the Proliferation of Vascular Smooth Muscle Cells.受体酪氨酸激酶：抑制血管平滑肌细胞增殖的仍然有趣的靶点。
Am J Cardiovasc Drugs. 2023 Sep;23(5):497-518. doi: 10.1007/s40256-023-00596-3. Epub 2023 Jul 31.
7
Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends.再生牙髓治疗中的神经再生：概述及当前趋势。
Int J Mol Sci. 2022 Dec 7;23(24):15492. doi: 10.3390/ijms232415492.
8
Non-alcoholic fatty liver disease and liver secretome.非酒精性脂肪性肝病与肝脏分泌组。
Arch Pharm Res. 2022 Dec;45(12):938-963. doi: 10.1007/s12272-022-01419-w. Epub 2022 Nov 28.
9
Molecular approaches for spinal cord injury treatment.脊髓损伤治疗的分子方法。
Neural Regen Res. 2023 Jan;18(1):23-30. doi: 10.4103/1673-5374.344830.
10
New developments in the biology of fibroblast growth factors.成纤维细胞生长因子生物学的新进展。
WIREs Mech Dis. 2022 Jul;14(4):e1549. doi: 10.1002/wsbm.1549. Epub 2022 Feb 9.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.对培养的小鼠胚胎细胞生长及其发育成既定细胞系的定量研究。
J Cell Biol. 1963 May;17(2):299-313. doi: 10.1083/jcb.17.2.299.
3
4
The initiation of cell division in a contact-inhibited mammalian cell line.接触抑制的哺乳动物细胞系中细胞分裂的起始
J Cell Physiol. 1965 Dec;66(3):325-33. doi: 10.1002/jcp.1030660310.
5
Migration of mouse 3T3 fibroblasts in response to a serum factor.小鼠3T3成纤维细胞对血清因子的迁移反应。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2799-801. doi: 10.1073/pnas.68.11.2799.
6
"Pleiotypic response".多型性反应
Nat New Biol. 1971 Aug;232(33):206-11.
7
Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts.正常及猿猴病毒40转化的3T3小鼠成纤维细胞的血清因子需求
Proc Natl Acad Sci U S A. 1971 Mar;68(3):645-52. doi: 10.1073/pnas.68.3.645.
8
"Contact inhibition" of cell division in 3T3 cells.3T3细胞中细胞分裂的“接触抑制”
Proc Natl Acad Sci U S A. 1968 May;60(1):300-4. doi: 10.1073/pnas.60.1.300.
9
Buffer combinations for mammalian cell culture.用于哺乳动物细胞培养的缓冲液组合。
Science. 1971 Oct 29;174(4008):500-3. doi: 10.1126/science.174.4008.500.
10
Stimulation of division of density inhibited fibroblasts by glucocorticoids.糖皮质激素对密度抑制的成纤维细胞分裂的刺激作用。
Nature. 1973 Apr 6;242(5397):399-401. doi: 10.1038/242399a0.
Zotero 插件