Armelin H A
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6. doi: 10.1073/pnas.70.9.2702.
Results are reported showing that pituitary extracts stimulate growth of 3T3 cells, an established line of mouse fibroblast. Cells were cultured in medium containing an organic buffer (HEPES; N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), which increases the serum requirement for cell growth. The pituitary growth-prompting activity is (a) not dialyzable; (b) thermolabile; (c) protease sensitive; and (d) enhanced by hydrocortisone (a steroid hormone). Activity was quantitatively assayed by measurement of stimulation of cell division or [(3)H]thymidine uptake into DNA. The active species is probably different from pituitary protein hormones and it seems to be effective at concentrations observed for protein hormones in vivo. 3T3 cells transformed by simian virus 40 are not responsive to this activity.
研究结果表明，垂体提取物能刺激3T3细胞（一种已建立的小鼠成纤维细胞系）的生长。细胞在含有有机缓冲剂（HEPES；N-2-羟乙基哌嗪-N'-2-乙磺酸）的培养基中培养，该缓冲剂会增加细胞生长对血清的需求。垂体的促生长活性具有以下特点：（a）不可透析；（b）对热不稳定；（c）对蛋白酶敏感；（d）氢化可的松（一种类固醇激素）可增强其活性。通过测量细胞分裂的刺激或[³H]胸苷掺入DNA来定量测定活性。活性物质可能与垂体蛋白激素不同，并且在体内观察到的蛋白激素浓度下似乎具有活性。被猿猴病毒40转化的3T3细胞对这种活性无反应。
