去神经支配大鼠膈肌肌肉中乙酰胆碱受体的合成
Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.
作者信息
Brockes J P, Hall Z W
出版信息
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1368-72. doi: 10.1073/pnas.72.4.1368.
Abstract
Acetylcholine receptor was purified by affinity chromatography from denervated rat hemidiaphragms that had been incubated in organ culture for 24 hr in medium containing [35-S] methionine. Radioactive acetylcholine receptor was identified in purified preparations by zone sedimentation in a sucrose gradient, by isoelectric focusing, and by precipitation with an antiserum to the acetylcholine receptor from electric eel. When innervated and denervated hemidiaphragms were incubated with [35-S] methionine in organ culture, and the acetylcholine receptors from each were purified separately, only the preparation from denervated muscles contained radioactive receptor as determined by zone sedimentation. We conclude that newly synthesized receptor is accumulated as a result of muscle denervation.
摘要
通过亲和层析从在含有[35-S]甲硫氨酸的培养基中进行器官培养24小时的去神经大鼠半膈肌中纯化乙酰胆碱受体。通过在蔗糖梯度中进行区带沉降、等电聚焦以及用电鳗抗乙酰胆碱受体抗血清沉淀，在纯化制剂中鉴定出放射性乙酰胆碱受体。当在器官培养中用[35-S]甲硫氨酸孵育有神经支配和去神经支配的半膈肌，并分别纯化各自的乙酰胆碱受体时，通过区带沉降测定，只有来自去神经肌肉的制剂含有放射性受体。我们得出结论，新合成的受体是肌肉去神经支配的结果而积累的。
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