培养的胚胎肌肉细胞分化过程中乙酰胆碱受体的合成
Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells.
作者信息
Merlie J P, Sobel A, Changeux J P, Gros F
出版信息
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4028-32. doi: 10.1073/pnas.72.10.4028.
Abstract
Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.
摘要
乙酰胆碱受体是特化肌膜的一个组成部分，在组织培养中胚胎肌原性细胞分化过程中出现。将放射性前体L-[35S]甲硫氨酸掺入纯化的受体多肽中，以此作为其从头合成的证据。纯化的放射性物质的身份通过[35S]受体与[3H]α-银环蛇毒素结合活性在蔗糖梯度上的共沉降以及与电鳗纯化乙酰胆碱受体抗血清的交叉反应来确定。
相似文献
1
Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells.培养的胚胎肌肉细胞分化过程中乙酰胆碱受体的合成
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4028-32. doi: 10.1073/pnas.72.10.4028.
2
Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.去神经支配大鼠膈肌肌肉中乙酰胆碱受体的合成
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1368-72. doi: 10.1073/pnas.72.4.1368.
3
Regulation of acetylcholine receptor by cyclic AMP.环磷酸腺苷对乙酰胆碱受体的调节作用。
J Biol Chem. 1980 Feb 25;255(4):1235-8.
4
Acetylcholine receptors in normal and denervated rat diaphragm muscle. II. Comparison of junctional and extrajunctional receptors.正常和去神经大鼠膈肌中的乙酰胆碱受体。II. 接头处和接头外受体的比较。
Biochemistry. 1975 May 20;14(10):2100-6. doi: 10.1021/bi00681a009.
5
Studies on purified eel acetylcholine receptor and anti-acetylcholine receptor antibody.纯化鳗鱼乙酰胆碱受体及抗乙酰胆碱受体抗体的研究。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3334-8. doi: 10.1073/pnas.70.12.3334.
6
Appearance of acetylcholine receptors in cultured myoblasts prior to fusion.融合前培养的成肌细胞中乙酰胆碱受体的出现。
J Supramol Struct. 1976;4(3):381-7. doi: 10.1002/jss.400040309.
7
Macromolecular characterization of muscle membranes. Acetylcholine receptor of normal and denervated muscle.肌肉膜的大分子特征。正常和去神经肌肉的乙酰胆碱受体。
J Biol Chem. 1974 Oct 10;249(19):6163-5.
8
Immunological studies of acetylcholine receptors.乙酰胆碱受体的免疫学研究。
J Supramol Struct. 1976;4(3):389-403. doi: 10.1002/jss.400040310.
9
The effect of calcium on acetylcholine receptor synthesis.钙对乙酰胆碱受体合成的影响。
J Neurosci. 1981 Jul;1(7):771-6. doi: 10.1523/JNEUROSCI.01-07-00771.1981.
10
Characterization of a nicotinic acetylcholine receptor from rabbit skeletal muscle and reconstitution in planar phospholipid bilayers.兔骨骼肌烟碱型乙酰胆碱受体的特性及其在平面磷脂双分子层中的重组。
Biochem Biophys Res Commun. 1976 Jan 26;68(2):577-84. doi: 10.1016/0006-291x(76)91184-0.
引用本文的文献
1
Switching of filamin polypeptides during myogenesis in vitro.体外成肌过程中细丝蛋白多肽的转换
J Cell Biol. 1983 Feb;96(2):321-9. doi: 10.1083/jcb.96.2.321.
2
Evidence for transcriptional regulation of the myosin heavy chain gene during myogenesis.肌生成过程中肌球蛋白重链基因转录调控的证据。
Nucleic Acids Res. 1984 Mar 26;12(6):2717-29. doi: 10.1093/nar/12.6.2717.
3
Skeletal muscle neural cell adhesion molecule (N-CAM): changes in protein and mRNA species during myogenesis of muscle cell lines.骨骼肌神经细胞黏附分子（N-CAM）：肌肉细胞系肌生成过程中蛋白质和mRNA种类的变化
J Cell Biol. 1987 Sep;105(3):1377-86. doi: 10.1083/jcb.105.3.1377.
4
Posttranslational control of membrane-skeleton (ankyrin and alpha beta-spectrin) assembly in early myogenesis.早期肌生成过程中膜骨架（锚蛋白和αβ-血影蛋白）组装的翻译后调控
J Cell Biol. 1985 May;100(5):1726-35. doi: 10.1083/jcb.100.5.1726.
5
Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression.小鼠肌肉烟碱型乙酰胆碱受体γ亚基：cDNA序列与基因表达
Nucleic Acids Res. 1986 Apr 25;14(8):3539-55. doi: 10.1093/nar/14.8.3539.
6
Activity-dependent regulation of gene expression in muscle and neuronal cells.肌肉和神经细胞中基因表达的活性依赖性调控。
Mol Neurobiol. 1989 Spring-Summer;3(1-2):1-53. doi: 10.1007/BF02935587.
7
Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers.发育中肌纤维膜上荧光标记的乙酰胆碱受体的横向运动。
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4594-8. doi: 10.1073/pnas.73.12.4594.
8
Protein synthesis and actin heterogeneity in calf muscle cells in culture.培养的小牛肌肉细胞中的蛋白质合成与肌动蛋白异质性
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2018-22. doi: 10.1073/pnas.73.6.2018.
9
Regulation of acetylcholine receptor levels by a cholinergic agonist in mouse muscle cell cultures.胆碱能激动剂对小鼠肌肉细胞培养物中乙酰胆碱受体水平的调节
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3488-92. doi: 10.1073/pnas.75.7.3488.
10
Loss of growth control and differentiation in the fu-1 variant of the L8 line of rat myoblasts.大鼠成肌细胞L8系fu-1变体中生长控制和分化的丧失。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3888-92. doi: 10.1073/pnas.74.9.3888.
本文引用的文献
1
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
3
Tritium labelling of the alpha-neurotoxin of Naja nigricollis.黑颈眼镜蛇α-神经毒素的氚标记
FEBS Lett. 1971 Oct 1;17(2):333-335. doi: 10.1016/0014-5793(71)80180-1.
4
Characterization of macromolecules by constant velocity sedimentation.通过等速沉降法对大分子进行表征。
Nature. 1967 Jul 22;215(5099):360-3. doi: 10.1038/215360a0.
5
The action of tubocurarine and atropine on the normal and denervated rat diaphragm.筒箭毒碱和阿托品对正常及去神经大鼠膈肌的作用。
J Physiol. 1967 Jan;188(1):53-66. doi: 10.1113/jphysiol.1967.sp008123.
6
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
7
Purification and properties of the cholinergic receptor protein from Electrophorus electricus electric tissue.电鳗电组织中胆碱能受体蛋白的纯化及特性
Eur J Biochem. 1974 Jun 15;45(2):371-94. doi: 10.1111/j.1432-1033.1974.tb03563.x.
8
Fate of alpha-bungarotoxin bound to acetylcholine receptors of normal and denervated muscle.与正常及失神经肌肉的乙酰胆碱受体结合的α-银环蛇毒素的命运
Science. 1974 Apr 26;184(4135):473-5. doi: 10.1126/science.184.4135.473.
9
Acetycholine receptor production and incorporation into membranes of developing muscle fibers.乙酰胆碱受体的产生及整合到发育中肌纤维的膜中。
Dev Biol. 1973 Jan;30(1):153-65. doi: 10.1016/0012-1606(73)90054-7.
10
The effect of actinomycin D on RNA and protein synthesis during differentiation of myoblasts of line L 6 E: the half-life of functional myosin messenger.放线菌素D对L 6 E系成肌细胞分化过程中RNA和蛋白质合成的影响：功能性肌球蛋白信使核糖核酸的半衰期
Biochimie. 1972;54(9):1157-67. doi: 10.1016/s0300-9084(72)80020-8.
Zotero 插件