Guyot C, Bouseta A, Scheirman V, Collin S
Unité de Brasserie et des Industries Alimentaires, Université Catholique de Louvain, Place Croix du Sud 2/Bte 7, B-1348 Louvain-la-Neuve, Belgium.
J Agric Food Chem. 1998 Feb 16;46(2):625-633. doi: 10.1021/jf970510l.
The apiculture industry is more and more interested in finding typical markers to authenticate floral origin of honeys. With this aim, some reliable volatile compounds were proposed to identify origin of lime tree and chestnut samples. A dichloromethane extraction followed by a Likens-Nickerson simultaneous steam distillation/solvent extraction led to representative honey extracts. About 400 volatile compounds were separated by gas chromatography, but only a few authenticated the floral origin of honeys. Chestnut honeys are distinguishable from other origins by high concentrations of acetophenone, 1-phenylethanol (>88 ppb), and 2-aminoacetophenone (>154 ppb). Lime tree honeys are characterized by enhanced amounts of shikimate pathway derivatives (ethylmethylphenol isomer (>31 ppb), 4-tert-butylphenol, estragole (>51 ppb), and p-methylacetophenone but also by high concentrations of monoterpene-derived compounds (menthol, thymol, 8-p-menthene-1,2-diol, and carvacrol (>76 ppb)) and methyl(1-methylethenyl)benzene.
养蜂行业越来越热衷于寻找典型标志物来鉴定蜂蜜的花源。出于这一目的，人们提出了一些可靠的挥发性化合物来鉴定椴树和栗子蜂蜜样本的来源。采用二氯甲烷萃取，随后进行利肯斯-尼克森同时蒸馏/溶剂萃取，得到具有代表性的蜂蜜提取物。通过气相色谱法分离出约400种挥发性化合物，但只有少数几种能够鉴定蜂蜜的花源。栗子蜂蜜与其他来源的蜂蜜的区别在于含有高浓度的苯乙酮、1-苯乙醇（>88 ppb）和2-氨基苯乙酮（>154 ppb）。椴树蜂蜜的特征在于莽草酸途径衍生物（乙基甲基苯酚异构体（>31 ppb）、4-叔丁基苯酚、草蒿脑（>51 ppb）和对甲基苯乙酮）含量增加，同时还含有高浓度的单萜衍生化合物（薄荷醇、百里香酚、8-p-薄荷烯-1,2-二醇和香芹酚（>76 ppb））以及甲基（1-甲基乙烯基）苯。
