Lee HJ, Choi GJ, Cho KY
Korea Research Institute of Chemical Technology, Jang-dong 100, Yusung-ku, P.O. Box 107, Taejon 305-606, Korea.
J Agric Food Chem. 1998 Feb 16;46(2):737-741. doi: 10.1021/jf970501c.
Dicarboximide fungicides iprodione, vinclozolin, and procymidone were examined for their capacity to inhibit mycelial growth, to cause cellular leakage, and to cause lipid peroxidation on Botrytis cinerea isolate BC2. All three fungicides effectively inhibited the mycelial growth of the fungi. The IC(50) values were found to be about 2 µM for all three fungicides, indicating that the fungicidal activity of the individual fungicides was almost the same. The fungicides caused significant cellular leakage and lipid peroxidation on the fungi in a concentration-dependent manner. Fungicidal activity of the three individual fungicides on inhibiting mycelial growth of the fungi correlated positively well with both cellular leakage and lipid peroxidation that were caused by the respective fungicides. Positive correlations were also found between the degree of cellular leakage and lipid peroxidation following treatment with the fungicides. Our results support the view that dicarboximide fungicides exert their fungicidal activity mainly through membrane lipid peroxidation and subsequent cellular leakage from the treated fungi.
研究了二甲酰亚胺类杀菌剂异菌脲、乙烯菌核利和腐霉利对灰葡萄孢菌分离株BC2的菌丝生长抑制能力、引起细胞渗漏及脂质过氧化的能力。这三种杀菌剂均能有效抑制该真菌的菌丝生长。发现这三种杀菌剂的IC(50)值均约为2 μM，表明各杀菌剂的杀真菌活性几乎相同。这些杀菌剂以浓度依赖的方式导致真菌显著的细胞渗漏和脂质过氧化。三种杀菌剂对真菌菌丝生长抑制的杀真菌活性与各自杀菌剂引起的细胞渗漏和脂质过氧化均呈良好的正相关。在用杀菌剂处理后，细胞渗漏程度与脂质过氧化之间也发现了正相关。我们的结果支持这样的观点，即二甲酰亚胺类杀菌剂主要通过膜脂质过氧化及随后处理真菌的细胞渗漏发挥其杀真菌活性。
