Griffiths Robert G, Dancer Jane, O'Neill Elizabeth, Harwood John L
School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK.
Bayer Crop Science, 14/20 rue Pierre Baizet, BP 9163, F-69263 Lyon, France.
New Phytol. 2003 Oct;160(1):199-207. doi: 10.1046/j.1469-8137.2003.00848.x.
• Botrytis cinerea is an important plant pathogen that causes grey mould in over 200 hosts. It is often controlled by dicarboximides, which have various proposed mechanisms of action, including effects on lipids. Here we have examined the effect of one dicarboximide, iprodione, on lipid metabolism. • B. cinerea, cultured in malt extract media, was challenged with iprodione and its lipids extracted, separated by TLC, and analysed by GLC. Lipid metabolism was followed using [1- C]acetate. • Triacylglycerol was the major nonpolar and phosphatidylcholine the main polar lipid in B. cinerea. Linoleate, followed by α-linolenate, were the major fatty acids and most lipid classes had compositions broadly similar to the total fatty acid pattern. Iprodione, at concentrations causing a cessation of growth (5 µM) caused a decrease in polar lipid but not total nonpolar lipid labelling. Within the nonpolar lipids, DAG was better labelled. • The data show that iprodione had a selective effect on lipid metabolism. The altered pattern of labelling suggested that choline (ethanolamine) phosphotransferase would be worth investigating as a primary site of action.
• 灰葡萄孢是一种重要的植物病原体，可在200多种寄主上引发灰霉病。它通常由二甲酰亚胺类杀菌剂控制，这类杀菌剂具有多种作用机制，包括对脂质的影响。在此，我们研究了一种二甲酰亚胺类杀菌剂异菌脲对脂质代谢的影响。
• 在麦芽提取物培养基中培养的灰葡萄孢，用异菌脲处理后提取其脂质，通过薄层层析（TLC）分离，并通过气相色谱（GLC）分析。使用[1-¹⁴C]乙酸追踪脂质代谢。
• 三酰甘油是灰葡萄孢中的主要非极性脂质，磷脂酰胆碱是主要的极性脂质。亚油酸之后是α-亚麻酸，它们是主要的脂肪酸，大多数脂质类别的组成与总脂肪酸模式大致相似。导致生长停止的浓度（5 μM）的异菌脲，使极性脂质标记减少，但不影响总非极性脂质标记。在非极性脂质中，二酰甘油的标记更好。
• 数据表明异菌脲对脂质代谢有选择性作用。标记模式的改变表明胆碱（乙醇胺）磷酸转移酶作为主要作用位点值得研究。
