Zeng Yaoying, Yu Qian, Cheng Shujun
College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, 510225, Guangdong, People's Republic of China.
Arch Microbiol. 2021 Sep;203(7):4025-4032. doi: 10.1007/s00203-021-02368-6. Epub 2021 May 26.
This study examined the antibacterial activity of the biological pesticide Liangguoan against Staphylococcus aureus and Escherichia coli as a potential replacement for chemical pesticide use in the fruit and vegetable industry. We measured the minimum inhibitory concentration and observed the changes in bacterial morphology, mortality, conductivity, nucleic acid content, and ATP content in response to the bactericide. The minimum inhibitory concentration of Liangguoan was 20 mg/mL for S. aureus and 40 mg/mL for E. coli. After treatment with Liangguoan, the mortality rates of S. aureus and E. coli reached 78.3% and 63.7%, respectively. We observed that the cells were scattered and that the cell morphology was altered in that the cells shortened. The interconnection effect and ATP content decreased, whereas cell conductivity and the nucleic acid content increased. In summary, Liangguoan inhibited S. aureus and E. coli by destroying their cell structure and disrupting their metabolism.
本研究检测了生物农药良国安对金黄色葡萄球菌和大肠杆菌的抗菌活性，以作为果蔬行业化学农药使用的潜在替代品。我们测定了最低抑菌浓度，并观察了杀菌剂作用下细菌形态、死亡率、电导率、核酸含量和ATP含量的变化。良国安对金黄色葡萄球菌的最低抑菌浓度为20mg/mL，对大肠杆菌为40mg/mL。用良国安处理后，金黄色葡萄球菌和大肠杆菌的死亡率分别达到78.3%和63.7%。我们观察到细胞分散，细胞形态发生改变，细胞变短。细胞间连接效应和ATP含量降低，而细胞电导率和核酸含量增加。总之，良国安通过破坏金黄色葡萄球菌和大肠杆菌的细胞结构并扰乱其代谢来抑制它们。
