谷胱甘肽、L-半胱氨酸和L-豆科氨酸对枯草芽孢杆菌ATCC 6633菌株中叠氮化物代谢产物合成及致突变性的影响。

Effect of glutathione L-cystein and L-djenkolic acid in the synthesis and mutagenicity of azide metabolite in Bacillus subtilis ATCC 6633 strain.

作者信息

Elbetieha A, Owais W M, Saadoun I, Hussein E

机构信息

Department of Biological Sciences, Faculty of Science, Jordan University of Science and Technology, Irbid, Jordan.

出版信息

New Microbiol. 1999 Oct;22(4):315-22.

PMID:10555201

Abstract

The Bacillus subtilis ATCC 6633 strain synthesizes a mutagenic metabolite from sodium azide and O-acetylserine. Mutagenicity of azide was decreased in growth media containing 10(-4) M glutathione, L-cysteine or L-djenkolic acid whereas dithiothritol (DTT) added at the same concentration did not reduce the mutagenicity of azide. Likewise, glutathione, L-cysteine, L-djenkolic acid, and DTT were found to have no effect in reducing the mutagenicity of the in vitro produced metabolite using bacterial cell-free extract. These results suggest that O-acetyl-serine sulfhydrylase catalyzes the reaction of azide and O-acetylserine to form a mutagenic metabolite, which is ninhydrin positive and migrates in TLC to an Rf value similar to that of azidoalanine in both acidic and basic solvent systems.

摘要

枯草芽孢杆菌ATCC 6633菌株可从叠氮化钠和O-乙酰丝氨酸合成一种诱变代谢物。在含有10⁻⁴ M谷胱甘肽、L-半胱氨酸或L-jenkolic酸的生长培养基中，叠氮化物的诱变性降低，而添加相同浓度的二硫苏糖醇（DTT）并未降低叠氮化物的诱变性。同样，发现谷胱甘肽、L-半胱氨酸、L-jenkolic酸和DTT在使用细菌无细胞提取物减少体外产生的代谢物的诱变性方面没有作用。这些结果表明，O-乙酰丝氨酸巯基酶催化叠氮化物与O-乙酰丝氨酸反应形成一种诱变代谢物，该代谢物茚三酮呈阳性，在薄层层析中迁移至Rf值，在酸性和碱性溶剂系统中均与叠氮丙氨酸相似。