Linz Bodo, Windsor Helen M, Gajewski John P, Hake Caylie M, Drautz Daniela I, Schuster Stephan C, Marshall Barry J
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, United States of America ; Center for Comparative Genomics and Bioinformatics, Pennsylvania State University, University Park, Pennsylvania, United States of America.
PLoS One. 2013 Dec 10;8(12):e82187. doi: 10.1371/journal.pone.0082187. eCollection 2013.
The human gastric pathogen Helicobacter pylori is usually acquired during childhood and, in the absence of treatment, chronic infection persists through most of the host's life. However, the frequency and importance of H. pylori transmission between adults is underestimated. Here we sequenced the complete genomes of H. pylori strains that were transmitted between spouses and analysed the genomic changes. Similar to H. pylori from chronic infection, a significantly high proportion of the determined 31 SNPs and 10 recombinant DNA fragments affected genes of the hop family of outer membrane proteins, some of which are known to be adhesins. In addition, changes in a fucosyltransferase gene modified the LPS component of the bacterial cell surface, suggesting strong diversifying selection. In contrast, virulence factor genes were not affected by the genomic changes. We propose a model of the genomic changes that are associated with the transmission and adaptation of H. pylori to a new human host.
人类胃部病原体幽门螺旋杆菌通常在儿童时期感染，若不进行治疗，慢性感染会在宿主的大部分生命中持续存在。然而，幽门螺旋杆菌在成年人之间传播的频率和重要性被低估了。在此，我们对配偶间传播的幽门螺旋杆菌菌株的完整基因组进行了测序，并分析了基因组变化。与慢性感染的幽门螺旋杆菌相似，在确定的31个单核苷酸多态性（SNP）和10个重组DNA片段中，有相当高比例影响了外膜蛋白hop家族的基因，其中一些已知是粘附素。此外，岩藻糖基转移酶基因的变化改变了细菌细胞表面的脂多糖成分，表明存在强烈的多样化选择。相比之下，毒力因子基因不受基因组变化的影响。我们提出了一个与幽门螺旋杆菌传播和适应新人类宿主相关的基因组变化模型。
