Hendricks K B, Wang B Q, Schnieders E A, Thorner J
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3202, USA.
Nat Cell Biol. 1999 Aug;1(4):234-41. doi: 10.1038/12058.
In metazoans, certain calmodulin-related calcium-binding proteins (recoverins, neurocalcins and frequenins) are found at highest levels in excitable cells, but their physiological roles are largely uncharacterized. Here we show that Saccharomyces cerevisiae contains a frequenin homologue, Frq1, and that its target is Pik1, a phosphatidylinositol-4-OH kinase. Frq1 binds to a conserved sequence motif in Pik1 outside Pik1's catalytic domain and stimulates its activity in vitro. N-myristoylated Frq1 may also assist in Pik1 localization.
在多细胞动物中，某些与钙调蛋白相关的钙结合蛋白（恢复蛋白、神经钙蛋白和频繁蛋白）在可兴奋细胞中含量最高，但其生理作用在很大程度上尚未明确。我们在此表明，酿酒酵母含有一种频繁蛋白的同源物Frq1，其作用靶点是磷脂酰肌醇-4-羟基激酶Pik1。Frq1与Pik1催化结构域之外的一个保守序列基序结合，并在体外刺激其活性。N-肉豆蔻酰化的Frq1也可能有助于Pik1的定位。
