Sidhu G S, Mani H, Gaddipati J P, Singh A K, Seth P, Banaudha K K, Patnaik G K, Maheshwari R K
Center for Combat and Life Sustainment Research, Uniformed Services University of Health Sciences, Bethesda, MD 20814, USA.
Wound Repair Regen. 1999 Sep-Oct;7(5):362-74. doi: 10.1046/j.1524-475x.1999.00362.x.
Tissue repair and wound healing are complex processes that involve inflammation, granulation and tissue remodeling. Interactions of different cells, extracellular matrix proteins and their receptors are involved in wound healing, and are mediated by cytokines and growth factors. Previous studies from our laboratory have shown that curcumin (diferuloylmethane), a natural product obtained from the rhizomes of Curcuma longa, enhanced cutaneous wound healing in rats and guinea pigs. In this study, we have evaluated the efficacy of curcumin treatment by oral and topical applications on impaired wound healing in diabetic rats and genetically diabetic mice using a full thickness cutaneous punch wound model. Wounds of animals treated with curcumin showed earlier re-epithelialization, improved neovascularization, increased migration of various cells including dermal myofibroblasts, fibroblasts, and macrophages into the wound bed, and a higher collagen content. Immunohistochemical localization showed an increase in transforming growth factor-beta1 in curcumin-treated wounds compared to controls. Enhanced transforming growth factor-beta1 mRNA expression in treated wounds was confirmed by in situ hybridization, and laser scan cytometry. A delay in the apoptosis patterns was seen in diabetic wounds compared to curcumin treated wounds as shown by terminal deoxynucleotidyl transferase-mediated deoxyuridyl triphosphate nick end labeling analysis. Curcumin was effective both orally and topically. These results show that curcumin enhanced wound repair in diabetic impaired healing, and could be developed as a pharmacological agent in such clinical settings.
组织修复和伤口愈合是复杂的过程，涉及炎症、肉芽形成和组织重塑。不同细胞、细胞外基质蛋白及其受体之间的相互作用参与伤口愈合，并由细胞因子和生长因子介导。我们实验室之前的研究表明，姜黄素（二阿魏酰甲烷），一种从姜黄根茎中获得的天然产物，可促进大鼠和豚鼠的皮肤伤口愈合。在本研究中，我们使用全层皮肤打孔伤口模型，评估了口服和局部应用姜黄素对糖尿病大鼠和遗传性糖尿病小鼠伤口愈合受损的治疗效果。用姜黄素治疗的动物伤口显示出更早的上皮再形成、改善的新生血管形成、包括真皮肌成纤维细胞、成纤维细胞和巨噬细胞在内的各种细胞向伤口床的迁移增加，以及更高的胶原蛋白含量。免疫组织化学定位显示，与对照组相比，姜黄素治疗的伤口中转化生长因子-β1增加。通过原位杂交和激光扫描细胞术证实了治疗伤口中转化生长因子-β1 mRNA表达增强。如末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记分析所示，与姜黄素治疗的伤口相比，糖尿病伤口的凋亡模式延迟。姜黄素口服和局部应用均有效。这些结果表明，姜黄素可促进糖尿病受损愈合中的伤口修复，并可开发成为此类临床环境中的一种药物。
