Devillard E, Newbold C J, Scott K P, Forano E, Wallace R J, Jouany J P, Flint H J
Rowett Research Institute, Bucksburn, Aberdeen, UK.
FEMS Microbiol Lett. 1999 Dec 1;181(1):145-52. doi: 10.1111/j.1574-6968.1999.tb08837.x.
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.
我们首次报道了一种参与植物细胞壁多糖降解的原生动物酶的克隆及特性分析。从瘤胃原生动物多泡多套胃虫构建了一个cDNA文库，并分离出一个表达木聚糖酶活性的稳定克隆。编码的酶属于糖苷水解酶家族11，系统发育分析表明，与革兰氏阳性菌的催化结构域相比，它与瘤胃中的其他纤维分解真核生物（厌氧真菌）的关系更为密切。
