Kalim Bushra, Ali Nazish Mazhar
Microbiology Laboratory, Department of Zoology, GCU, Lahore, Pakistan.
3 Biotech. 2016 Dec;6(2):122. doi: 10.1007/s13205-016-0445-3. Epub 2016 Jun 3.
Efficiency of cellulase-free xylanases is one of the determining factors in paper and pulp industries. Use of microbes which can produce cellulase-free xylanases may help to overcome the current challenges in kraft pulp processing. Isolation and screening of microorganisms from local samples offers a possibility for obtaining the potential microbes for this purpose. This research was therefore aimed to collect, screen, characterize and identify potential cellulase-free xylanase producers. A total of 313 microbial isolates were collected while using selective media (EBAM and XAM) to determine the xylanolytic potential of microbes. Qualitative and quantitative analyses were performed and finally 11 bacterial and 6 fungal strains were selected for characterization and identification. The potential isolates were identified as Bacillus pumilus (388.82 U/mg), Bacillus safensis (385.26 U/mg), Aspergillus flavus (493.33 U/mg) and Aspergillus niger (419.33 U/mg). Optimization of the microbial strains while using agro-industrial waste is suggested.
无纤维素酶木聚糖酶的效率是造纸和纸浆工业的决定因素之一。使用能够产生无纤维素酶木聚糖酶的微生物可能有助于克服硫酸盐制浆过程中当前面临的挑战。从当地样本中分离和筛选微生物为获得用于此目的的潜在微生物提供了可能性。因此，本研究旨在收集、筛选、表征和鉴定潜在的无纤维素酶木聚糖酶产生菌。在使用选择性培养基（EBAM和XAM）测定微生物的木聚糖分解潜力时，共收集了313株微生物分离株。进行了定性和定量分析，最终选择了11株细菌和6株真菌菌株进行表征和鉴定。潜在分离株被鉴定为短小芽孢杆菌（388.82 U/mg）、沙福芽孢杆菌（385.26 U/mg）、黄曲霉（493.33 U/mg）和黑曲霉（419.33 U/mg）。建议在使用农业工业废料时对微生物菌株进行优化。
