Mve-Obiang A, Remacle J, Palomino J C, Houbion A, Portaels F
Laboratoire de Biochimie et Biologie Cellulaire, Facultés Universitaires Notre Dame de la Paix, Rue de Bruxelles 61, B-5000, Namur, Belgium.
FEMS Microbiol Lett. 1999 Dec 1;181(1):153-7. doi: 10.1111/j.1574-6968.1999.tb08838.x.
The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein-free growth conditions.
致病性生长缓慢的溃疡分枝杆菌，直到现在，一直是在含有白蛋白的液体培养基中培养。在此，我们首次报道了使用苏通培养基和改良里德培养基这两种无蛋白培养基的情况，在这两种培养基中溃疡分枝杆菌能够生长并产生其毒素，这种毒素是这种环境微生物的一种主要毒力因子，该微生物会引发一种通常被称为布鲁里溃疡的皮肤病。这些结果表明，苏通培养基和改良里德培养基可能对溃疡分枝杆菌研究中某些需要无蛋白生长条件的领域有用。
