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Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro.

作者信息

Borisy G G, Marcum J M, Olmsted J B, Murphy D B, Johnson K A

出版信息

Ann N Y Acad Sci. 1975 Jun 30;253:107-32. doi: 10.1111/j.1749-6632.1975.tb19196.x.

DOI:10.1111/j.1749-6632.1975.tb19196.x
PMID:1056738
Abstract
摘要

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Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro.从猪脑中纯化微管蛋白及相关高分子量蛋白质并对体外微管组装进行表征
Ann N Y Acad Sci. 1975 Jun 30;253:107-32. doi: 10.1111/j.1749-6632.1975.tb19196.x.
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The role of rings in the assembly of microtubules in vitro.
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Separation of active tubulin and microtubule-associated proteins by ultracentrifugation and isolation of a component causing the formation of microtubule bundles.通过超速离心分离活性微管蛋白和微管相关蛋白,并分离出一种导致微管束形成的成分。
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Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.高分子量蛋白质与微管的关联及其在体外微管组装中的作用。
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