Shirabe K, Shimada M, Kajiyama K, Hasegawa H, Gion T, Ikeda Y, Takenaka K, Sugimachi K
Second Department of Surgery, Faculty of Medicine, Kyushu University, Japan.
Surgery. 1999 Nov;126(5):842-6.
Matrix metalloproteinase-9 (MMP-9) has recently been reported to be related to cancer cell invasion. This study was performed to clarify the expression of MMP-9 in surgically resected intrahepatic cholangiocarcinoma (IHCC).
In 37 patients with IHCC who underwent a surgical resection, the expression of MMP-9 and the clinicopathologic characteristics of MMP-9-positive IHCC were investigated. The expression of MMP-9 was immunohistochemically detected in 16 (43%) of 37 IHCC. The patients were divided into MMP-9 (-) IHCC (n = 21), MMP-9 (+) IHCC (n = 12), and MMP-9 (++) IHCC (n = 4).
The survival rate after surgical resection in patients with MMP-9 (-), (+), and (++) IHCC, was 66%, 39%, and 0% at one year, 50%, 32% and 0% at 3 years, respectively (P = .001). The incidence rate of lymph node metastasis was 6 (28%) of 21 in MMP-9 (-) patients, 7 (58%) of 12 in MMP-9 (+) patients and 4 (100%) of 4 in MMP-9 (++) patients. The incidence rate of lymph node metastasis increased in proportion to an increase in the expression of MMP-9 in IHCC (P = .02). Recurrence in the lymph node was more common in patients with MMP-9 (+) and (++) cancers than in those with MMP-9 (-) cancers.
The expression of MMP-9 in IHCC was a prognostic factor related to lymph node metastasis.
最近有报道称基质金属蛋白酶-9（MMP-9）与癌细胞侵袭有关。本研究旨在阐明MMP-9在手术切除的肝内胆管癌（IHCC）中的表达情况。
对37例行手术切除的IHCC患者，研究MMP-9的表达及MMP-9阳性IHCC的临床病理特征。37例IHCC中16例（43%）通过免疫组化检测到MMP-9的表达。患者分为MMP-9（-）IHCC组（n = 21）、MMP-9（+）IHCC组（n = 12）和MMP-9（++）IHCC组（n = 4）。
MMP-9（-）、（+）和（++）IHCC患者术后1年生存率分别为66%、39%和0%，3年生存率分别为50%、32%和0%（P = .001）。MMP-9（-）患者21例中有6例（28%）发生淋巴结转移，MMP-9（+）患者12例中有7例（58%）发生淋巴结转移，MMP-9（++）患者4例中有4例（100%）发生淋巴结转移。IHCC中淋巴结转移发生率随MMP-9表达增加而升高（P = .02）。MMP-9（+）和（++）癌患者的淋巴结复发比MMP-9（-）癌患者更常见。
IHCC中MMP-9的表达是与淋巴结转移相关的预后因素。
