[基于动态界面张力测定数据的实验性粉尘诱导支气管炎中的肺表面活性物质状态]

[Pulmonary surfactant status in experimental dust-induced bronchitis based on the data from dynamic interphase tensiometry].

作者信息

Siniachenko O V, Korzh E V, Kazakov V N, Belokon' A M, Shcherbakov K S, Vladzimirskiĭ A V

出版信息

Med Tr Prom Ekol. 1999(10):21-5.

Abstract

The disease was simulated by inhalation of dust containing 29% of free silica in Wistar male rats during 8 months. One group of the subjects was kept under 26-28 degrees C and other one--under 38-40 degrees C. Dynamic surface tension of lung tissue homogenate was studied by means of computer tensiometer MRT (Lauda, Germany). Interphase tensiometry precisely characterizes state of lung surfactant in experimental chronic dust bronchitis. Equilibrium surface tension and surface tension for 0.01 and 1 sec deteriorate during combined exposure to silica dust and heating microclimate. The studies demonstrated strong correlation between interphase tensiogram parameters and individual lipids content of lung surfactant. Surface tension is related to the state of lipids peroxidation in lungs and antioxidant systems.

摘要

通过在8个月内让Wistar雄性大鼠吸入含有29%游离二氧化硅的粉尘来模拟该疾病。一组实验对象饲养在26 - 28摄氏度的环境中，另一组饲养在38 - 40摄氏度的环境中。使用德国劳达公司的计算机张力计MRT研究肺组织匀浆的动态表面张力。相间张力测定法精确地表征了实验性慢性尘支气管炎中肺表面活性剂的状态。在二氧化硅粉尘和热微气候联合作用下，平衡表面张力以及0.01秒和1秒时的表面张力会恶化。研究表明相间张力图参数与肺表面活性剂的个别脂质含量之间存在很强的相关性。表面张力与肺中脂质过氧化状态和抗氧化系统有关。