Origgi F C, Klein P A, Mathes K, Blahak S, Marschang R E, Tucker S J, Jacobson E R
Department of Small Animal Clinical Sciences, University of Florida, Gainesville, Florida 32610-0126, USA.
J Clin Microbiol. 2001 Sep;39(9):3156-63. doi: 10.1128/JCM.39.9.3156-3163.2001.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in Mediterranean tortoises [spur-thighed tortoise (Testudo graeca) and Hermann's tortoise (Testudo hermanni)]. This serodiagnostic test was validated through a hyperimmunization study. The mean of the A(405) readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used as the cutoff for seropositivity in tortoises. ELISA results were compared to serum neutralization (SN) values for the same samples by using the McNemar test. The results obtained by SN and ELISA were not significantly different (P > 0.05). This new ELISA could be used as an important diagnostic tool for screening wild populations and private and zoo collections of Mediterranean tortoises.
开发了一种酶联免疫吸附测定(ELISA),用于检测与地中海陆龟[ spur - thighed tortoise(Testudo graeca)和 Hermann's tortoise(Testudo hermanni)]上呼吸道疾病相关的疱疹病毒抗体。通过超免疫研究验证了这种血清学诊断测试。将超免疫研究零时采集的血浆样本的 A(405)读数平均值加上三倍标准差用作陆龟血清阳性的临界值。通过 McNemar 检验将 ELISA 结果与相同样本的血清中和(SN)值进行比较。SN 和 ELISA 获得的结果无显著差异(P > 0.05)。这种新的 ELISA 可作为筛查地中海陆龟野生种群以及私人和动物园收藏的重要诊断工具。