由钙离子螯合剂乙二醇双四乙酸（EGTA）诱导的大肠杆菌中的不规则核定位和无核细胞产生。

Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA.

作者信息

Wachi M, Iwai N, Kunihisa A, Nagai K

机构信息

Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku Yokohama 226-8501, Japan.

出版信息

Biochimie. 1999 Aug-Sep;81(8-9):909-13. doi: 10.1016/s0300-9084(99)00204-7.

DOI:10.1016/s0300-9084(99)00204-7

PMID:10572305

Abstract

A screening system for specific inhibitors of chromosome partitioning in Escherichia coli was constructed using the blue assay method developed for detection of anucleate cell production. Effects of known chemical compounds and antibiotics were examined in the system. It was found that a calcium-chelating reagent, EGTA, induced blue zones around the paper disks containing EGTA at concentrations which did not induce growth inhibition zones. Induction of anucleate cell production by EGTA was confirmed by fluorescence microscopy after DAPI staining. Even in the nucleated cells, irregular intracellular localization of nucleoids was frequently observed. The effect of EGTA was reversed by addition of Ca(2+). These results suggest the possible role of calcium ion in the process of chromosome partitioning in E. coli.

摘要

利用为检测无核细胞产生而开发的蓝色检测方法，构建了一种用于筛选大肠杆菌中染色体分配特异性抑制剂的系统。在该系统中检测了已知化合物和抗生素的作用。发现一种钙螯合剂乙二醇双四乙酸（EGTA），在不诱导生长抑制圈的浓度下，能在含有EGTA的滤纸片周围诱导产生蓝色区域。经4′,6-二脒基-2-苯基吲哚（DAPI）染色后通过荧光显微镜观察，证实了EGTA可诱导无核细胞的产生。即使在有核细胞中，也经常观察到类核在细胞内的定位不规则。添加Ca(2+)可逆转EGTA的作用。这些结果表明钙离子在大肠杆菌染色体分配过程中可能发挥作用。