Kim Heui-Soo, Takenaka Osamu, Crow Timothy J
POWIC, Department of Psychiatry, Warneford Hospital, University of Oxford, Oxford OX3 7JX, UK1.
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484, Japan 2.
J Gen Virol. 1999 Oct;80 ( Pt 10):2613-2619. doi: 10.1099/0022-1317-80-10-2613.
An investigation was undertaken of primate pol gene sequences from a novel endogenous retrovirus family, ERV-W, related to a new human endogenous retrovirus family (HERV-W) that includes multiple sclerosis-associated retrovirus (MSRV) sequences identified in particles recovered from monocyte cultures from patients with multiple sclerosis. The pol gene sequences of the ERV-W family were detected in hominoids and Old World monkeys, but not in New World monkeys, whereas ERV-W long terminal repeat-like elements were detected in all primates (hominoids, Old World monkeys and New World monkeys). Thirty-two pol gene sequences from hominoids and Old World monkeys showed a high degree of sequence identity to MSRV and other HERV-W sequences. Phylogenetic analysis indicated close relationships of pol gene sequences across primate species. The analysis suggests that the ERV-W family has evolved independently but in constrained patterns ('parallel evolution') in different primate species, including man. The ratio of synonymous to non-synonymous substitutions indicated that negative selective pressure is acting on CHW1-1 from chimpanzee, HBW6-6 from baboon and HWX5 from man, sequences that have no disruption by point mutation or insertions/deletions. Therefore, these pol gene sequences could be associated with an active provirus in primates. The findings indicate that the ERV-W family has continued to evolve in the course of the primate radiation and may include members with a capacity to influence gene function and possibly cause disease.
对来自一个新的内源性逆转录病毒家族ERV-W的灵长类动物pol基因序列进行了研究，该家族与一个新的人类内源性逆转录病毒家族（HERV-W）相关，后者包括在从多发性硬化症患者的单核细胞培养物中回收的颗粒中鉴定出的多发性硬化症相关逆转录病毒（MSRV）序列。ERV-W家族的pol基因序列在类人猿和旧世界猴中被检测到，但在新世界猴中未被检测到，而ERV-W长末端重复样元件在所有灵长类动物（类人猿、旧世界猴和新世界猴）中均被检测到。来自类人猿和旧世界猴的32个pol基因序列与MSRV和其他HERV-W序列显示出高度的序列同一性。系统发育分析表明不同灵长类物种的pol基因序列关系密切。分析表明，ERV-W家族在包括人类在内的不同灵长类物种中独立进化，但以受限模式（“平行进化”）进行。同义替换与非同义替换的比率表明，负选择压力作用于来自黑猩猩的CHW1-1、来自狒狒的HBW6-6和来自人类的HWX5，这些序列没有因点突变或插入/缺失而中断。因此，这些pol基因序列可能与灵长类动物中的活性前病毒有关。研究结果表明，ERV-W家族在灵长类动物辐射过程中持续进化，可能包括具有影响基因功能并可能导致疾病能力的成员。
